Full
Outline
The gene expression in Hericium coralloides on the process of producing γ-aminobutyric acid (GABA) was investigated under Schisandra chinensis （Turcz.）Baill. induction environment at different growth stages. H. coralloides RT25 was used in this research. The H. coralloides RT25 growth state of the fermentation mycelium and the primordium, the various indicators of the fruiting body were compared and analyzed between the experimental group with Schisandra and the control group without Schisandra. Transcriptome sequencing technology was used to analyze the differentially expressed genes and related enrichment pathways of H. coralloides at different growth and development stages. The results showed that the fermentation broth mycelial pellets of the Schisandra group had higher uniformity and density than those of the control group. The fruiting body of the Schisandra group showed significant improvement in mycelium growth rate, primordium formation and biological efficiency. Transcriptome study showed that the DN344 gene expression of H. coralloides RT25 with Schisandra group was up-regulated during the mycelium fermentation stage, and the activity of glutamic acid dehydrogenase was enhanced, resulting in the increase of L-glutamic acid, thereby promoting the increase of GABA content. The DN3464 gene expression of H. coralloides RT25 with Schisandra induction was up-regulated in the primordium formation period, and the activity of glutamic acid decarboxylase was improved, leading to improve the biological synthesis efficiency of GABA. The DN344 gene expression was up-regulated in the fruiting body maturity, the glutamic acid dehydrogenase activity was enhanced, and the L-glutamic acid content increased while the expression level of DN3464 gene and the activity of glutamic acid decarboxylase remained stable, and the activity of γ-aminobutyric acid transaminase was enhanced, which finally led to the increase of GABA content. Schisandra regulates the signal transduction pathway in cells, affects the expression of the related genes for GABA synthesis, and then regulates the activity of enzymes related to the GABA metabolic pathway, promotes the conversion of glutamic acid to GABA, finally improves the yield of GABA.
121
33
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
