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In order to improve the yield of total flavonoids from fruiting body of Sanghuangporus vaninii, and to evaluate biological activity of the total flavonoids, the ultrasonic-assisted method was used to extract total flavonoids from raw material of S. vaninii fruiting body with the yield of total flavonoids as indicator, the extraction process was optimized using response surface methodology, and the hypoglycemic activity and antioxidant activity were determined. The results showed that the optimal extraction process of S. vaninii total flavonoids was with 60％ethanol as solvent, liquid-solid ratio of 42∶1 mL/g , extraction time of 2.7 h, and extraction temperature of 75 ℃, and the yield of S. vaninii total flavonoids was （49.31±0.45） mg/g, and the total antioxidant capacity of S. vaninii total flavonoids through the experiments in vitro are good. The IC50 of S. vaninii total flavonoids for scavenging DPPH free radicals and hydroxyl free radicals were （1.31±0.06） μg/mL and （73.62±1.50） μg/mL, respectively. The results of hypoglycemic activity analysis in vitro showed that IC50 of S. vaninii total flavonoids for inhibiting α-glucosidase and α-amylase were （0.042±0.002）μg/mL and （0.324±0.083）μg/mL with both of competitive-noncompetitive mixed inhibition, respectively. The extraction process of S. vaninii total flavonoids is simple and feasible to conduct, and S. vaninii total flavonoids have good antioxidant and hypoglycemic activity.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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