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Untargeted metabolomics analysis using a UHPLC-Exploris240 system was used to analyze intracellular metabolites and regulatory mechanisms between the high erythritol-producing mutant and wild-type strain of Yarrowia lipolytica during fermentation. A total of 482 significantly differential metabolites were identified. KEGG pathway enrichment analysis revealed that the pathways related to erythritol biosynthesis of this strain were significantly enriched, involving the pathways related to carbon flux utilization, which also preliminarily revealed that carbon flux redistribution may be a key regulatory mechanism affecting the increase of erythritol production of this mutant strain. In addition, the study also showed that the enhancement of the cofactor had a synergistic effect on erythritol synthesis in this mutant strain. Further analysis revealed that the intracellular content of glycerol, which can serve as a non-oxidative carbon source, significantly increased in the mutant strain. Subsequent fermentation experiments further demonstrated that under mixed carbon source (glycerol/glucose) conditions, the mutant strain achieved a higher erythritol yield of 73.67 g/L. This indicates that appropriate exogenous glycerol has a certain promoting effect on erythritol synthesis in the mutant strain. Thus, this study provides insights into the metabolic regulation of erythritol synthesis in Y. lipolytica at the metabolite level through metabolomic analysis, and also offers a basis for the rational strain modification via metabolic engineering strategies to enhance erythritol production.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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