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In Situ Enrichment of Actinoplanes Using Polyaspartic Acid Water Retaining Agent and Screening of Its Secondary Metabolic Potential
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Pei-zhi DING1, 2, Hong-ling SHEN1, 2, Qing-tang DAI1, Han GAO1, Xing-xin HUANG1, Chuan-xing WAN1, 2, *
Journal of Microbiology | 2026, 46(4) : 42 - 53
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Journal of Microbiology | 2026, 46(4): 42-53
In Situ Enrichment of Actinoplanes Using Polyaspartic Acid Water Retaining Agent and Screening of Its Secondary Metabolic Potential
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Pei-zhi DING1, 2, Hong-ling SHEN1, 2, Qing-tang DAI1, Han GAO1, Xing-xin HUANG1, Chuan-xing WAN1, 2, *
Affiliations
  • 1.College of Life Sciences and Technology, Tarim University, Alar 843300
  • 2.State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, Tarim University, Alar 843300
Published: 2026-08-20 doi: 10.3969/j.issn.1005-7021.2026.04.004
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The Tarim River Basin is rich in microbial resources. To explore the species diversity of Actinoplanes in its soil, uncover new Actinoplanes resources, and enrich the germplasm resource bank of the Tarim River Basin, the in situ enrichment of Actinoplanes was achieved using polyaspartic acid water-retaining agent (PASP). The fermentation crude extracts of Actinoplanes were screened for antimicrobial activity using seven pathogenic bacteria as target strains. This was combined with antiSMASH gene cluster analysis and detection of antibiotic biosynthesis genes. The results indicate that 244 strains isolated and identified from soil samples enriched in situ with PASP belong to 3 phyla, 5 classes, 4 orders, 11 families, 16 genera, and 59 species, respectively. Among these, 228 strains belong to the phylum Actinomycetota, accounting for 93%. The genus Actinoplanes exhibited a high isolation rate, with 103 isolated strains and 21 species, representing 42%. Seven strains of Actinoplanes with both antimicrobial activity and metabolic potential were screened. Soil samples with high moisture content along the Tarim River harbor abundant rare actinobacterial resources. The PASP in situ enrichment method can selectively isolate Actinoplanes, thereby facilitating further research on the diversity of rare actinobacteria in the soil microorganisms of the Tarim River Basin and on the exploration and utilization of Actinoplanes resources.

polyaspartic acid, PASP  /  Actinoplanes  /  enrichment culturing in situ  /  biodiversity  /  antibacterial activity
丁培治, 沈红玲, 代庆堂, 高晗, 黄星欣, 万传星. 聚天冬氨酸保水剂原位富集游动放线菌及其次级代谢潜力筛选. 微生物学杂志, 2026 , 46 (4) : 42 -53 . DOI: 10.3969/j.issn.1005-7021.2026.04.004
Pei-zhi DING, Hong-ling SHEN, Qing-tang DAI, Han GAO, Xing-xin HUANG, Chuan-xing WAN. In Situ Enrichment of Actinoplanes Using Polyaspartic Acid Water Retaining Agent and Screening of Its Secondary Metabolic Potential[J]. Journal of Microbiology, 2026 , 46 (4) : 42 -53 . DOI: 10.3969/j.issn.1005-7021.2026.04.004
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Year 2026 volume 46 Issue 4
84
16
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Article Info
doi: 10.3969/j.issn.1005-7021.2026.04.004
  • Receive Date：2025-12-08
  • Online Date：2026-10-10
  • Published：2026-08-20
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  • Received：2025-12-08
Funding
Affiliations
    1.College of Life Sciences and Technology, Tarim University, Alar 843300
    2.State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, Tarim University, Alar 843300
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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