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Outline
This study aimed to prepare monoclonal antibodies against human β2-MG and to establish rapid and accurate quantitative and qualitative immunoassay methods for β2-MG detection. Recombinant β2-MG was produced through prokaryotic expression and purification, and subsequently used for immunizing BALB/c mice. Monoclonal antibodies against human β2-MG were generated using hybridoma technology and screened via double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), which facilitated the development of a chemiluminescence enzyme immunoassay (CLEIA). Clinical serum β2-MG levels were measured to evaluate and analyze the detection performance of the proposed CLEIA. Additionally, a convenient and rapid colloidal gold immunochromatographic assay for β2-MG detection was preliminarily established. The monoclonal antibody pair 5H7/6H9-AP was developed into the CLEIA, demonstrating a linear range of 0.14-120.00 μg/L (extended to 60.00 mg/L). The serum β2-MG CLEIA results showed excellent correlation with the latex enhanced immunoturbidimetry assay conducted in hospitals (R2: 0.96, P<0.000 1). The established colloidal gold immunochromatographic assay exhibited good sensitivity for detecting recombinant β2-MG, with test strips capable of detecting a minimum concentration of 0.20 mg/L. The prepared monoclonal antibodies for β2-MG, along with the established quantitative and qualitative immunoassay methods, hold significant potential for clinical diagnostic applications.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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