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The cytoskeleton could regulate the fusion and division of mitochondria, meanwhile, mitochondria also could regulate the normal dynamics of the cytoskeleton. The mpc1 gene encodes mitochondrial pyruvate carrier 1 which is located in the mitochondrial membrane. The protein of MPC1 is an essential component of the mitochondrial pyruvate carrier complexes. Currently, the effect of mpc1 gene deletion on the dynamics of spindle, histone, and actin has not been reported. In this study, fluorescent labels of microtubules, histone, and actin were constructed in Schizosaccharomyces pombe cells. The stability of cell dynamics at different stages was observed using live-cell imaging to investigate the effects of mpc1 gene deletion on actin. Compared with the wild-type strain, the results showed that the mpc1Δ strain reduced the spindle elongation time by 6.70 min (P<0.01), accelerated the elongation rate by 0.17μm/min (P<0.01), reduced the chromosome segregation time by 3.40 min (P<0.01), and prolonged the total time for actin ring aggregation and contraction by 3.80 min (P<0.01) at room temperature. The experimental results mentioned above indicated that although the deletion of mpc1 gene promoted spindle elongation and chromosome segregation, it slowed down the formation and contraction of actin rings, ultimately leading to slow cell growth. This study provided a basis for further investigation of mpc1 function.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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