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To reduce nitrogen loss during aerobic composting, enhance compost fertility, and minimize environmental pollution, this study used cattle manure as the material to isolate a highly efficient heterotrophic nitrifying strain of NB-2. Through morphological characterization and 16S rRNA gene sequence analysis, the strain was identified as Alcaligenes faecalis. Further research showed that NB-2 exhibited significantly enhanced ammonia oxidation capacity under the conditions of sodium citrate as the carbon source, C/N ratio of 15, temperature of 35 ℃, and pH of 9.0. The ammonia nitrogen () removal rate reached 100% at 32 hours, with a removal rate as high as 33.38 mg/(L·h). This study demonstrated the potential of strain NB-2 in composting applications to reduce nitrogen losses while improving fertilizer quality and efficiency, thereby promoting the sustainable development of the livestock industry.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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