In this study, fungal diversity was analyzed and antimicrobial activity isolates were screened from Eling Lake to enrich the fungi diversity in the extreme environment and to provide basic materials for subsequent development of biocontrol agents. Ten sediment samples were collected from shoreside of Eling Lake, and fungi were isolated by dilution separation method using seven selective mediums. The isolated fungi were identified by morphological characteristics and rDNA-ITS sequence phylogenetic analysis, and antimicrobial activity isolates were screened by confront culture method. One hundred and forty-four fungi isolates were obtained, belonging to totally 22 genera of Ascomycota, Mucoromycota and Zygomycota. Seven high antimicrobial activity isolates were screened from one hundred and forty-four fungi isolates, they had a broad spectrum of antimicrobial activity on eight fungi pathogens from maize, highland barley and wheat. Moreover, they were Phaeosphaeria and Emericellopsis, respectively. These results showed that sediment fungi resource in Eling Lake was abundant and have certain biocontrol and utilization value.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |