Home Journals Articles Updates About
CN
image
收藏切换
Structures, functions, and directed evolution of microbial carbon-fixing enzymes: from catalytic mechanisms to engineering applications
收藏切换
PDF
Zhiyao PENG1, 2, Kaixing XIAO1, 2, Xia FANG1, 2, Jikai ZONG1, 2, Yunyan YUAN1, 2, Yanting YU1, 2, Shanquan LIANG1, 2, Qinhong WANG3, 4, 5, *, Dan WANG1, 2, *
Acta Microbiologica Sinica | 2026, 66(9) : 4299 - 4319
Less
收藏切换
Acta Microbiologica Sinica | 2026, 66(9): 4299-4319
• Review •
Structures, functions, and directed evolution of microbial carbon-fixing enzymes: from catalytic mechanisms to engineering applications
Full
Zhiyao PENG1, 2, Kaixing XIAO1, 2, Xia FANG1, 2, Jikai ZONG1, 2, Yunyan YUAN1, 2, Yanting YU1, 2, Shanquan LIANG1, 2, Qinhong WANG3, 4, 5, *, Dan WANG1, 2, *
Affiliations
  • 1.Chongqing Key Laboratory of Low-Carbon Synthetic Bio-Manufacturing, Chongqing, China
  • 2.School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, China
  • 3.Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China
  • 4.National Engineering Research Center of Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China
  • 5.National Center of Technology Innovation for Synthetic Biology, Tianjin, China
Published: 2026-09-04 doi: 10.13343/j.cnki.wsxb.20260414
Outline
收藏切换

In the context of global carbon emission reduction and carbon neutrality strategies, microbial carbon-fixing enzymes have emerged as a research frontier in biological carbon fixation technologies due to their unique advantages, including high efficiency, low energy consumption, and the ability to produce value-added chemicals. This review systematically summarizes the classification, structural characteristics, catalytic mechanisms, and regulatory networks of both natural and engineered carbon-fixing enzymes. It focuses on the roles and kinetic regulation of key enzymes such as ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), carbonic anhydrase (CA), formolase (FLS), and carbon monoxide dehydrogenase/acetyl-CoA synthase (CODH/ACS) in CO2 fixation. From a structural biology perspective, the review discusses how static structures and dynamic conformational changes determine catalytic efficiency. Furthermore, this paper reviews the recent advances in protein engineering strategies including rational design, directed evolution, high-throughput screening, and artificial intelligence (AI)-assisted prediction for carbon-fixing enzymes, along with the enabling role of specialized databases such as Greenase in enzyme mining and pathway reconstruction. Representative case studies, including RuBisCO, CA, FLS, and multi-enzyme cascade systems, are adopted to illustrate engineering pathways from single-enzyme optimization to integrated systems. Finally, future directions are discussed, including dynamic catalytic mechanism simulation, in vivo fitness engineering, de novo enzyme design, and electro-/photo-enzyme coupling systems. This review provides a theoretical reference for the development of efficient, stable, and industrially adaptable novel biological carbon fixation technologies.

carbon-fixing enzyme  /  carbon dioxide  /  carbon-fixing enzyme database  /  AI-assisted design  /  directed evolution
Zhiyao PENG, Kaixing XIAO, Xia FANG, Jikai ZONG, Yunyan YUAN, Yanting YU, Shanquan LIANG, Qinhong WANG, Dan WANG. Structures, functions, and directed evolution of microbial carbon-fixing enzymes: from catalytic mechanisms to engineering applications[J]. Acta Microbiologica Sinica, 2026 , 66 (9) : 4299 -4319 . DOI: 10.13343/j.cnki.wsxb.20260414
Funding
Less
  • the Key Research and Development Project of Key Areas in Guangdong Province(2024B1111160005)
  • the National Key Research and Development Program of China(2022YFC2105700)
  • the National Natural Science Foundation of China(22378032)
  • the Chongqing Human Resources and Social Security Bureau Project(cx2023036)
Appendix
Less
Year 2026 volume 66 Issue 9
PDF
119
54
Cite this Article
BibTeX
Article Info
doi: 10.13343/j.cnki.wsxb.20260414
  • Receive Date：2026-05-16
  • Online Date：2026-09-09
  • Published：2026-09-04
Article Data
Affiliations
History
  • Received：2026-05-16
  • Accepted：2026-06-28
Funding
the Key Research and Development Project of Key Areas in Guangdong Province(2024B1111160005)
the National Key Research and Development Program of China(2022YFC2105700)
the National Natural Science Foundation of China(22378032)
the Chongqing Human Resources and Social Security Bureau Project(cx2023036)
Affiliations
    1.Chongqing Key Laboratory of Low-Carbon Synthetic Bio-Manufacturing, Chongqing, China
    2.School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, China
    3.Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China
    4.National Engineering Research Center of Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China
    5.National Center of Technology Innovation for Synthetic Biology, Tianjin, China

Corresponding:

*E-mail: WANG Qinhong,
WANG Dan,
References
Share
https://castjournals.cast.org.cn/joweb/wswxb/EN/10.13343/j.cnki.wsxb.20260414
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House