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Litopenaeus vannamei is one of the crustacean species with the highest production and economic value in the global aquaculture industry. Its health status is closely linked to the community succession and balance of the microbiota in the aquaculture system, and the stable supply of seeds is one of the fundamental and core components of the high-quality development of the shrimp aquaculture industry. Characterizing the pattern of bacterial community succession during the early developmental stages of shrimp is an essential prerequisite for achieving microbiome-based regulation in larviculture. [Objective] To establish a standardized bacterial community dataset covering the entire cycle of L. vannamei larviculture, thereby providing data support for systematic investigations on the microbial communities during shrimp larvae development. [Methods] Focusing on the complete developmental stages of L. vannamei larvae, larval shrimps and rearing water samples were collected from the larviculture system of the Zhejiang Mariculture Research Institute following standardized sampling and laboratory processing protocols. Bacterial community datasets were constructed by high-throughput 16S rRNA gene amplicon sequencing combined with the standard Dix-seq amplicon analysis pipeline. [Results] This dataset encompassed the raw paired-end sequencing reads from 102 samples (48 larval samples and 54 rearing water samples) across the entire cycle of shrimp larviculture, comprising a total of 204 fastq.gz files and 4 709 988 raw paired-end reads. After quality control, 4 255 994 effective sequences and 202 505 zero-radius operational taxonomic units (ZOTUs) were obtained. Data generation strictly followed standardized protocols, with multi-point composite sampling ensuring sample representativeness of microbial samples, and unified sample collection and nucleic acid extraction procedures were employed. All analytical parameters were publicly disclosed through the parameter card mechanism of the Dix-seq pipeline, guaranteeing the reproducibility and reliability of the data analyses. [Conclusion] The samples in this dataset cover the key developmental nodes of L. vannamei larvae, achieving synchronous monitoring of the bacterial communities throughout the entire larviculture period. It provides fundamental data for exploring bacterial community succession and host-environment interaction mechanisms during the early developmental stages of shrimp, thereby facilitating the development of green larviculture technologies based on microbiome regulation. Additionally, it serves as a benchmark for the standardized management and analysis of similar high-throughput sequencing data, which is of great significance for enhancing the standardization and operability of microbiome research in agricultural ecosystems.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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