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[Objective] To investigate the effects of luxS deletion on the biofilm formation and stress tolerance of Listeria monocytogenes and to elucidate the role of luxS in regulating environmental adaptability of this pathogen. [Methods] With Listeria monocytogenes ATCC 19112 as the parental strain, a luxS-deleted mutant, a complemented strain, and an empty-vector control strain were constructed. Growth curve analysis, environmental stress tolerance assays and biofilm formation assays were performed to comparatively analyze phenotypic differences among the wild-type, mutant, and complemented strains. [Results] Compared with the wild-type strain, the luxS-deleted mutant exhibited reduced adaptability under adverse conditions, including low temperature, high osmotic pressure, oxidative stress, and acid stress. Its biofilm-forming ability decreased after 24 h and 48 h of incubation (P<0.01), accompanied by a reduction in the production of extracellular polymeric substances in the biofilm matrix. Further transcriptional analysis revealed that the expression levels of agrA, agrB, lmo2504, and sigB were downregulated (P<0.05), whereas that of ladR was upregulated (P<0.05) following luxS deletion. Under disinfectant stress, MTT assay showed that the metabolic activity of the luxS-deleted strain was lower than that of the wild-type and complemented strains after 48 h of biofilm cultivation (P<0.05). After 72 h, no significant differences were observed among the strains. [Conclusion] These findings demonstrate that luxS plays a critical role in regulating the environmental adaptability of L. monocytogenes and provide a novel theoretical basis for the development of future control and prevention strategies targeting this pathogen.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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