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[Objective] To investigate the efficacy of postbiotics prepared with Bifidobacterium animalis CCFM1155, a strain previously identified for its ability to enhance nuclear factor erythroid 2-related factor 2 (Nrf2) secretion in human immortalized epidermal cells, in alleviating skin aging. [Methods] A mouse model of skin aging was established by daily subcutaneous injection of D-galactose (500 mg/kg) for eight weeks (n=6 per group). The model mice were then orally administered either heat-inactivated cells (1155B) or concentrated fermentation supernatant (1155T) of CCFM1155 cells at a dose equivalent to 5×109 CFU of live bacteria. [Results] The 1155B was the primary postbiotic component exerting anti-skin aging effects, whereas the 1155T group showed no significant effect. Compared with the model group, the 1155B treatment ameliorated skin aging phenotypes by improving the skin tissue structure, increasing the epidermal thickness (P<0.05) and dermal collagen fiber density (P<0.01), and decreasing the expression of the senescence marker protein p21 and the activity of senescence-associated β-galactosidase (P<0.001). In addition, the 1155B treatment enhanced the antioxidant defense system of the skin by elevating the activities of total superoxide dismutase (P<0.05) and catalase (P<0.01), as well as the content of reduced glutathione (P<0.01), while lowering the malondialdehyde level (P<0.01). Mechanism studies revealed that the 1155B upregulated both the mRNA (P<0.05) and protein (P<0.001) levels of Nrf2 in the skin. Consequently, it promoted the transcription of key downstream antioxidant molecules, including heme oxygenase-1, peroxiredoxin, thioredoxin 1, and the glutamate-cysteine ligase catalytic subunit, thereby activating the downstream signaling pathway of Nrf2. [Conclusion] The inactivated cells of B. animalis CCFM1155 alleviates skin aging by activating the Nrf2 signaling pathway. These findings offer a novel perspective on postbiotic regulation of the Nrf2 pathway and lay a theoretical foundation for developing safe and effective anti-skin aging products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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