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Isolation, identification, and pathogenicity analysis of a porcine deltacoronavirus strain CHN/GSYD/2023
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Ruiming YU1, Liping ZHANG2, Peng ZHOU2, Zhongwang ZHANG2, Li PAN2, Huichen GUO2, Ligang YUAN1, Xinsheng LIU2
Acta Microbiologica Sinica | 2026, 66(8) : 3994 - 4007
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Acta Microbiologica Sinica | 2026, 66(8): 3994-4007
• Research Article •
Isolation, identification, and pathogenicity analysis of a porcine deltacoronavirus strain CHN/GSYD/2023
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Ruiming YU1, Liping ZHANG2, Peng ZHOU2, Zhongwang ZHANG2, Li PAN2, Huichen GUO2, Ligang YUAN1, Xinsheng LIU2
Affiliations
  • 1.College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu, China
  • 2.State Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy for Agricultural Sciences, Lanzhou, Gansu, China
Published: 2026-08-04 doi: 10.13343/j.cnki.wsxb.20260128
Outline
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Porcine deltacoronavirus (PDCoV), a major porcine intestinal coronavirus, has caused enormous economic losses to the pig industry. Up to now, there is no commercial vaccine available. [Objective] To obtain the epidemic strain of PDCoV and study its biological characteristics and pathogenicity, thereby providing biological materials for the development of an effective vaccine against PDCoV as soon as possible. [Methods] LLC-PK1 cells were used to isolate the virus from PDCoV-positive samples from a pig farm in Gansu Province. The virus was identified by observation of cytopathic effect (CPE), indirect immunofluorescence assay (IFA), and whole genome sequencing. [Results] After blind passage of the PDCoV-positive LLC-PK1 cells to P6, the cells became larger and rounded, and then shrunk and shed into single or clustered dense particles, typical features of CPE. The IFA results confirmed successful isolation of the PDCoV strain CHN/GSYD/2023. The whole genome sequencing and phylogenetic analysis showed that CHN/GSYD/2023 isolated in this study was more closely related to the strain from China and was located on a different clade from the strain CHN/XJYN/2016 preserved in our laboratory. After 5-day-old piglets were infected with CHN/GSYD/2023, 100% (5/5) of piglets became diseased. Autopsy and histopathology of piglets showed thinning small intestinal wall, a large amount of effusion in the intestinal cavity, ileum intestinal villus necrosis and other pathological damage. [Conclusion] A PDCoV strain CHN/GSYD/2023 was successfully isolated from PDCoV-positive samples in a pig farm in Gansu. The strain can be stably proliferated and passaged in LLC-PK1 cells, and it is highly pathogenic to suckling piglets.

porcine deltacoronavirus  /  isolation and identification  /  pathogenicity
Ruiming YU, Liping ZHANG, Peng ZHOU, Zhongwang ZHANG, Li PAN, Huichen GUO, Ligang YUAN, Xinsheng LIU. Isolation, identification, and pathogenicity analysis of a porcine deltacoronavirus strain CHN/GSYD/2023[J]. Acta Microbiologica Sinica, 2026 , 66 (8) : 3994 -4007 . DOI: 10.13343/j.cnki.wsxb.20260128
Funding
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  • National Center of Technology Innovation for Pigs Project(NCTIP-XD/C 03)
  • Major Science and Technology Project of Gansu Province(24ZD13NA008)
  • Major Science and Technology Project of Gansu Province(23ZDNA007)
Appendix
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Year 2026 volume 66 Issue 8
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Article Info
doi: 10.13343/j.cnki.wsxb.20260128
  • Receive Date：2026-02-10
  • Online Date：2026-08-21
  • Published：2026-08-04
Article Data
Affiliations
History
  • Received：2026-02-10
  • Accepted：2026-03-25
Funding
National Center of Technology Innovation for Pigs Project(NCTIP-XD/C 03)
Major Science and Technology Project of Gansu Province(24ZD13NA008)
Major Science and Technology Project of Gansu Province(23ZDNA007)
Affiliations
    1.College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu, China
    2.State Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy for Agricultural Sciences, Lanzhou, Gansu, China

Corresponding:

E-mail: YUAN Ligang, ;
LIU Xinsheng,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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