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Objective Cyanocobalamin (CN-CbI) requires to be converted into adenosylcobalamin (Ado-CbI) to exert neuroprotective effects, yet its conversion efficiency is impaired under conditions such as chronic sleep deprivation (CSD). This study aimed to obtain a bacterial strain with high efficiency in converting CN-CbI to Ado-CbI that could enhance neuroprotective effects. Methods Lactobacillus paragasseri CCFM1526, a strain capable of converting CN-CbI to Ado-CbI, was isolated via UPLC. A mouse model of CSD was established, and the cognitive functions of mice were evaluated by the novel object recognition and Morris water maze tests. The neuronal structure in the hippocampal dentate gyrus (DG) region was observed via histological staining. Tissue vitamin B12 levels were measured, and the ERK/mTOR signaling pathway along with related neural protein expression was analyzed to assess the neuroprotective mechanism of the fermentation broth. Results Compared with CN-CbI supplementation alone, the fermentation broth of L. paragasseri CCFM1526 significantly improved the cognitive function and alleviated the structural damage in the hippocampal DG region of CSD mice. Intervention with the fermentation broth increased the total vitamin B12 content in the liver, serum, and brain by 10.2%, 16.3%, and 29.0%, respectively (P<0.05). Meanwhile, it activated the ERK/mTOR signaling pathway, leading to increases of 21.5%, 52.4%, 17.3%, and 19.7% in the content of myelin basic protein, postsynaptic density protein 95, brain-derived neurotrophic factor, and nerve growth factor, respectively (P<0.05). Conclusion L. paragasseri CCFM1526 converts CN-CbI into Ado-CbI through fermentation, subsequently activating the ERK/mTOR signaling pathway and upregulating the expression of neurotrophic and myelin repair-related proteins, thereby alleviating CSD-induced nerve injury.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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