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Objective In view of the issues of reservoir acidification and pipeline corrosion caused by sulfate-reducing bacteria (SRB) during oil and gas field development, this study focused on the green synthesis of silver nanoparticles with bamboo leaf extract and systematically evaluated their effectiveness and mechanism in inhibiting SRB. Methods Under alkaline conditions (pH 11.0) and at 80 ℃, silver nanoparticles with a particle size of 20-50 nm and good monodispersity were successfully prepared through ultrasonically assisted ethanol extraction of active substances from bamboo leaves. Results Real-time quantitative PCR results showed that 50 μg/mL of silver nanoparticles reduced the total bacterial count from 5.21×109 copies/mL to 2.01×107 copies/mL. Meanwhile, the abundance of sulfate-reducing functional genes dsrB and aprA decreased from 1.76×109 copies/mL and 2.03×109 copies/mL to undetectable levels. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay indicated that silver nanoparticles reduced SRB activity in a dose-dependent manner, with the SRB activity in the 50 μg/mL silver nanoparticles group decreasing to 40% of that in the control group. The lactate dehydrogenase (LDH) release assay confirmed that the cytotoxic effect of the synthesized silver nanoparticles ranged from 32.8% to 42.1%. Under SEM/TEM, silver nanoparticles were observed to adsorb onto the cell membrane surface, forming a nanoscale coating that altered membrane permeability and disrupted the cell wall structure. At a concentration of 50 μg/mL, silver nanoparticles completely inhibited biofilm formation. Core simulation experiments further validated the effective inhibition of SRB by the nanoparticles in reservoir environments, with hydrogen sulfide production decreasing from 83.16 mg/L to below the limit of detection. Conclusion This study demonstrates that bamboo leaf-mediated synthesized silver nanoparticles exhibit high efficiency in inhibiting SRB, environmental friendliness, and resistance to microbial drug resistance. It provides a green prevention and control strategy for microbial corrosion in oil and gas development processes.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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