Home Journals Articles Updates About
CN
image
收藏切换
Role of extracellular polymeric substances in regulation of dissimilatory iron reduction and hydroxyl radical generation in Shewanelladecolorationis
收藏切换
PDF
Yunqi HUANG1, 2, Yi WANG1, Wenwen AN2, Cuifen GAN2, Shaofeng ZHOU2, Meiying XU2
Acta Microbiologica Sinica | 2026, 66(6) : 3088 - 3104
Less
收藏切换
Acta Microbiologica Sinica | 2026, 66(6): 3088-3104
• Research Article •
Role of extracellular polymeric substances in regulation of dissimilatory iron reduction and hydroxyl radical generation in Shewanelladecolorationis
Full
Yunqi HUANG1, 2, Yi WANG1, Wenwen AN2, Cuifen GAN2, Shaofeng ZHOU2, Meiying XU2
Affiliations
  • 1.Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, Institute of Environmental Health and Pollution Control, Guangdong Provincial Engineering Technology Research Center for Solid Waste Recycling and Safe Treatment, School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou, Guangdong, China
  • 2.State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, Guangdong, China
Published: 2026-06-04 doi: 10.13343/j.cnki.wsxb.20260193
Outline
收藏切换

Objective Microbial-Fenton process driven by dissimilatory iron reduction is increasingly recognized as a major source of hydroxyl radicals (•OH) in redox-fluctuating environments (e.g., tidal sediments), thereby playing an important role in biogeochemical element cycling. However, extracellular polymeric substances (EPS), which are ubiquitous and closely associated with the cell-mineral interface, remain poorly understood in terms of their regulatory roles in this process. This study aims to elucidate the mechanisms by which EPS derived from Shewanella decolorationis influence •OH generation under oxic-anoxic conditions. Methods S. decolorationis S12, its extracellular electron transfer-deficient mutants (S12ΔBA and S12ΔccmA), extracted EPS, and ferrihydrite were employed as model components. By simulating oxic-anoxic alternating conditions, we employed a combination of chemical and spectroscopic approaches to characterize the physicochemical properties of EPS and to investigate their effects on iron reduction and •OH generation. Results Although EPS exhibited intrinsic redox activity and could mediate electron transfer in S. decolorationis, they exerted inhibitory effects on iron reduction efficiency and •OH generation under oxic-anoxic conditions, decreasing the Fe(Ⅱ) accumulation and •OH production by up to (56.63±4.67)% and (26.86±5.30)%, respectively. This inhibition was primarily attributed to the strong affinity between EPS and iron minerals, which led to the formation of EPS-Fe(Ⅲ) complexes that hindered electron transfer efficiency. In addition, EPS promoted the transformation of ferrihydrite into secondary iron mineral phases with lower bioavailability, thereby decreasing the reducibility of Fe(Ⅲ) and further suppressing •OH generation. Conclusion EPS act as a critical interfacial chemical mediator in the microbe-iron mineral system, regulating dissimilatory iron reduction and consequently influencing •OH production. These findings provide new insights into the biogeochemical processes in tidal soil and water environments such as intertidal sediments.

Shewanella decolorationis  /  extracellular polymeric substances  /  microbial-Fenton process  /  extracellular electron transfer  /  dissimilatory iron reduction
Yunqi HUANG, Yi WANG, Wenwen AN, Cuifen GAN, Shaofeng ZHOU, Meiying XU. Role of extracellular polymeric substances in regulation of dissimilatory iron reduction and hydroxyl radical generation in Shewanelladecolorationis[J]. Acta Microbiologica Sinica, 2026 , 66 (6) : 3088 -3104 . DOI: 10.13343/j.cnki.wsxb.20260193
Funding
Less
  • the National Natural Science Foundation of China(42377132)
  • the National Natural Science Foundation of China(U24A20637)
  • the National Natural Science Foundation of China(42377242)
  • the National Natural Science Foundation of China(41907122)
  • the GDAS’ Special Project of Science and Technology Development(2024GDASZH-2024010102)
  • the Young Talent Project of GDAS(2024GDASQNRC-0201)
  • the Guangdong Special Support Plan for Outstanding Talents(2023JC07L096)
  • the Guangdong Special Support Program for Young Talents(2024TQ08A621)
  • the Guangdong Rural Science and Technology Commissioner Project(STKJ2025040)
  • the Guangzhou Science and Technology Program(2025A04J5265)
Appendix
Less
Year 2026 volume 66 Issue 6
PDF
22
9
Cite this Article
BibTeX
Article Info
doi: 10.13343/j.cnki.wsxb.20260193
  • Receive Date：2026-03-09
  • Online Date：2026-06-17
  • Published：2026-06-04
Article Data
Affiliations
History
  • Received：2026-03-09
  • Accepted：2026-04-10
Funding
the National Natural Science Foundation of China(42377132)
the National Natural Science Foundation of China(U24A20637)
the National Natural Science Foundation of China(42377242)
the National Natural Science Foundation of China(41907122)
the GDAS’ Special Project of Science and Technology Development(2024GDASZH-2024010102)
the Young Talent Project of GDAS(2024GDASQNRC-0201)
the Guangdong Special Support Plan for Outstanding Talents(2023JC07L096)
the Guangdong Special Support Program for Young Talents(2024TQ08A621)
the Guangdong Rural Science and Technology Commissioner Project(STKJ2025040)
the Guangzhou Science and Technology Program(2025A04J5265)
Affiliations
    1.Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, Institute of Environmental Health and Pollution Control, Guangdong Provincial Engineering Technology Research Center for Solid Waste Recycling and Safe Treatment, School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou, Guangdong, China
    2.State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, Guangdong, China

Corresponding:

E-mail: WANG Yi,
ZHOU Shaofeng,
References
Share
https://castjournals.cast.org.cn/joweb/wswxb/EN/10.13343/j.cnki.wsxb.20260193
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House