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Objective The outer membrane protein CirA serves as a specific transporter for catecholate-type siderophores and is involved in the uptake of siderophores and other nutrients, playing a crucial role in bacterial physiology. However, its impact on bacterial antibiotic resistance remains unclear. This study aimed to investigate the role of ahcirA in the antibiotic resistance of Aeromonas hydrophila ATCC 7966 under antibiotic stress, thereby providing a theoretical basis for elucidating the molecular mechanism by which ahcirA regulates bacterial resistance. Methods With A. hydrophila ATCC 7966 as the model organism, an ahcirA knockout strain (ΔahcirA) was constructed, and its susceptibility to multiple quinolones and aminoglycosides was assessed. Quantitative proteomics was further employed to compare protein expression profiles of ΔahcirA with and without antibiotic stress. Bioinformatic approaches were adopted for the functional analysis of differentially expressed proteins. Results In the media containing enrofloxacin and norfloxacin, the growth of ΔahcirA was significantly impaired compared with that of the wild-type strain. In contrast, ΔahcirA exhibited enhanced growth in the media supplemented with kanamycin and streptomycin. Proteomic and bioinformatic analyses revealed that the deletion of ahcirA may alter bacterial antibiotic resistance by affecting the expression of proteins involved in multiple biological processes, such as small molecule metabolism, and by modulating the expression of antibiotic resistance genes. Conclusion CirA plays a significant role in the antibiotic resistance of A. hydrophila. Its absence influences bacterial susceptibility to different classes of antibiotics by regulating the expression of diverse functional proteins and antibiotic resistance genes.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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