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Advances in microbial isolation and screening technologies
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Hongyu MA1, Yewei JIN1, Miao LI1, Yuna LI1, Wei HUA1, 2, Shuang WU1, 2, Yanling CHENG1, 2, Wanqing WANG1, 2, Na ZHANG1, 2, Cheng ZHOU1, 2
Acta Microbiologica Sinica | 2026, 66(5) : 2133 - 2147
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Acta Microbiologica Sinica | 2026, 66(5): 2133-2147
• Review •
Advances in microbial isolation and screening technologies
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Hongyu MA1, Yewei JIN1, Miao LI1, Yuna LI1, Wei HUA1, 2, Shuang WU1, 2, Yanling CHENG1, 2, Wanqing WANG1, 2, Na ZHANG1, 2, Cheng ZHOU1, 2
Affiliations
  • 1.College of Biochemical Engineering, Beijing Union University, Beijing, China
  • 2.Beijing Key Laboratory of Biomass Waste Resource Utilization, Beijing Union University, Beijing, China
Published: 2026-05-04 doi: 10.13343/j.cnki.wsxb.20250930
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Microorganisms represent the largest untapped resource reservoir on the Earth, and breakthroughs in their isolation and cultivation are prerequisites for fundamental advances in the life sciences. This review focuses on recent progress in the isolation and screening technologies for bacteria, fungi, and archaea. It systematically elucidates how the development and application of cutting-edge isolation and screening technologies have enhanced the efficiency of isolating previously uncultivable and rare microbial taxa. By summarizing lineage-specific strategies—such as multi-omics targeting and single-cell precision localization for bacteria, metabolomics-guided screening and microfluidic technology for fungi, and co-culture systems coupled with extreme-condition cultivation for archaea—this review highlights the core value of interdisciplinary technology integration in bridging genomic data with in situ functional validation. Finally, the article prospectively addresses challenges in data integration and the construction of automated workflows, thereby outlining a strategic pathway for the systematic exploration of microbial resources.

isolation and screening  /  bacteria  /  fungi  /  archaea
Hongyu MA, Yewei JIN, Miao LI, Yuna LI, Wei HUA, Shuang WU, Yanling CHENG, Wanqing WANG, Na ZHANG, Cheng ZHOU. Advances in microbial isolation and screening technologies[J]. Acta Microbiologica Sinica, 2026 , 66 (5) : 2133 -2147 . DOI: 10.13343/j.cnki.wsxb.20250930
Funding
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  • The National Key Research and Development Program of China(2022YFC3902403)
Appendix
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Year 2026 volume 66 Issue 5
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Article Info
doi: 10.13343/j.cnki.wsxb.20250930
  • Receive Date：2025-12-15
  • Online Date：2026-05-09
  • Published：2026-05-04
Article Data
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History
  • Received：2025-12-15
  • Accepted：2026-03-03
Funding
The National Key Research and Development Program of China(2022YFC3902403)
Affiliations
    1.College of Biochemical Engineering, Beijing Union University, Beijing, China
    2.Beijing Key Laboratory of Biomass Waste Resource Utilization, Beijing Union University, Beijing, China

Corresponding:

E-mail: ZHANG Na, ;
ZHOU Cheng,
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Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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