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Preparation of qualitative reference material for positive serum specific for genotype Ⅱ African swine fever virus strain HLJ/18
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Liyuan SHI1, 2, Yuanmao ZHU3, Yuan ZHANG2, Ying MU1, 2, Huaiyu JIANG1, 2, Jiamei YE2, 4, Jingjing ZHANG2, Mengqi ZHOU2, Xin HE2, Jianqiang NI2, Xiaoxue GU2, Encheng SUN3, Chuanbin WANG2, Yuliang LIU2, Dongming ZHAO3, Qi LI2
Acta Microbiologica Sinica | 2026, 66(5) : 2535 - 2546
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Acta Microbiologica Sinica | 2026, 66(5): 2535-2546
• Technology and Method •
Preparation of qualitative reference material for positive serum specific for genotype Ⅱ African swine fever virus strain HLJ/18
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Liyuan SHI1, 2, Yuanmao ZHU3, Yuan ZHANG2, Ying MU1, 2, Huaiyu JIANG1, 2, Jiamei YE2, 4, Jingjing ZHANG2, Mengqi ZHOU2, Xin HE2, Jianqiang NI2, Xiaoxue GU2, Encheng SUN3, Chuanbin WANG2, Yuliang LIU2, Dongming ZHAO3, Qi LI2
Affiliations
  • 1.College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China
  • 2.China Animal Disease Control Center, Beijing, China
  • 3.Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
  • 4.College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China
Published: 2026-05-04 doi: 10.13343/j.cnki.wsxb.20250862
Outline
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Objective To prepare reference material (RM) for positive serum specific for genotype Ⅱ African swine fever virus (ASFV) for serological detection, quality control, and proficiency testing (PT). Methods The anti-serum collected from specific pathogen-free (SPF) swine immunized with inactivated genotype Ⅱ ASFV was used as raw material for the preparation of RM. Indirect enzyme-linked immunosorbent assay (iELISA) was employed to evaluate the purity, specificity, homogeneity, and stability of RM. In addition, RM was characterized by nine laboratories and applied in clinical trials by three laboratories. Results A total of five hundred bottles of RM for positive serum specific for genotype Ⅱ ASFV strain HLJ/18 were successfully prepared. The results indicated that the RM we prepared was pure, homogenous, and free of exogenous virus contamination, showing good specificity, homogeneity, and stability. The RM was stable for at least 18 months when it was stored at -20 ℃ and for at least 7 days at 4 ℃, 25 ℃, and 37 ℃. The characterization by the nine laboratories showed that the RM was positive for antibodies against genotype Ⅱ ASFV. Conclusion The positive serum specific for genotype Ⅱ ASFV strain HLJ/18 has successfully been prepared, providing critical material for ASF detection and diagnosis.

genotype Ⅱ African swine fever virus  /  reference material  /  positive serum  /  homogeneity  /  stability  /  characterization
Liyuan SHI, Yuanmao ZHU, Yuan ZHANG, Ying MU, Huaiyu JIANG, Jiamei YE, Jingjing ZHANG, Mengqi ZHOU, Xin HE, Jianqiang NI, Xiaoxue GU, Encheng SUN, Chuanbin WANG, Yuliang LIU, Dongming ZHAO, Qi LI. Preparation of qualitative reference material for positive serum specific for genotype Ⅱ African swine fever virus strain HLJ/18[J]. Acta Microbiologica Sinica, 2026 , 66 (5) : 2535 -2546 . DOI: 10.13343/j.cnki.wsxb.20250862
Funding
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  • The National Key Research and Development Program of China(2024YFD1800300)
  • The Self-funded Project of China Animal Disease Control Center (CADC)(CADC2023303)
Appendix
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Year 2026 volume 66 Issue 5
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96
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Article Info
doi: 10.13343/j.cnki.wsxb.20250862
  • Receive Date：2025-11-18
  • Online Date：2026-05-09
  • Published：2026-05-04
Article Data
Affiliations
History
  • Received：2025-11-18
  • Accepted：2025-12-18
Funding
The National Key Research and Development Program of China(2024YFD1800300)
The Self-funded Project of China Animal Disease Control Center (CADC)(CADC2023303)
Affiliations
    1.College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China
    2.China Animal Disease Control Center, Beijing, China
    3.Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
    4.College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China

Corresponding:

E-mail: ZHAO Dongming, ;
LI Qi,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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