Home Journals Articles Updates About
CN
image
收藏切换
Role of the β-1,3-glucan-binding protein gene in Apis cerana cerana against Ascosphaera apis infection
收藏切换
PDF
Fangji WANG1, Xinrui CHEN1, Yaqin GAO1, Yuwei ZHANG1, Genchao GAN1, Shuai ZHOU1, Rui GUO1, 2, 3, Jianfeng QIU1, 2, 3, Zhongmin FU1, 2, 3, Dafu CHEN1, 2, 3
Acta Microbiologica Sinica | 2026, 66(5) : 2393 - 2403
Less
收藏切换
Acta Microbiologica Sinica | 2026, 66(5): 2393-2403
• Research Article •
Role of the β-1,3-glucan-binding protein gene in Apis cerana cerana against Ascosphaera apis infection
Full
Fangji WANG1, Xinrui CHEN1, Yaqin GAO1, Yuwei ZHANG1, Genchao GAN1, Shuai ZHOU1, Rui GUO1, 2, 3, Jianfeng QIU1, 2, 3, Zhongmin FU1, 2, 3, Dafu CHEN1, 2, 3
Affiliations
  • 1.College of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China
  • 2.National & Local United Engineering Laboratory of Natural Biotoxin, Fuzhou, Fujian, China
  • 3.Apitherapy Research Institute of Fujian Agriculture and Forestry University, Fuzhou, Fujian, China
Published: 2026-05-04 doi: 10.13343/j.cnki.wsxb.20250837
Outline
收藏切换

Objective To analyze the expression profile of the β-glucan-binding protein (Acβ-GBP) gene of Apis cerana cerana in response to Ascosphaera apis infection and to investigate the impacts of Acβ-GBP knockdown on the larval mortality and the incidence of chalkbrood disease following A. apis infection. These findings will provide a foundation for further functional research. Methods The sequence and structural characteristics of Acβ-GBP were analyzed via bioinformatics approaches. RT-qPCR was employed to investigate the expression profiles of Acβ-GBP in the larval midgut following A. apis infection. Furthermore, RNA interference (RNAi) was utilized to explore the impacts of Acβ-GBP on the larval mortality and the incidence of chalkbrood disease. Results The CDS length of Acβ-GBP was 1 440 bp, encoding a protein with a molecular weight of 54.68 kDa and a grand average of hydropathicity value of -0.22, which contained a typical transmembrane domain and signal peptide. Phylogenetic analysis revealed that β-GBP of A. c. cerana, Apis florea and Apis dorsata clustered into a single major clade. After A. apis infection, the expression level of Acβ-GBP in the midgut of A. c. cerana worker larvae was downregulated at 1-3 days post-infection (dpi) (P<0.05). Following RNA interference (RNAi)-mediated silencing of Acβ-GBP, its expression level was lower than that in the ds-egfp group at 2 and 3 dpi (P<0.01). The cumulative larval mortality and the incidence of chalkbrood disease both increased over the infection time, and the overall mortality was higher than that of the control group (P<0.000 1). Conclusion Acβ-GBP was capable of responding to A. apis infection, and knockdown of Acβ-GBP expression significantly impaired the resistance of honeybee larvae to A. apis. Collectively, β-GBP acts as an important immune recognition protein in A. c. cerana, and plays an important role in defending against fungal invasion.

Apis cerana cerana  /  β-1,3-glucan-binding protein  /  Ascosphaera apis  /  immune response  /  RNA interference
Fangji WANG, Xinrui CHEN, Yaqin GAO, Yuwei ZHANG, Genchao GAN, Shuai ZHOU, Rui GUO, Jianfeng QIU, Zhongmin FU, Dafu CHEN. Role of the β-1,3-glucan-binding protein gene in Apis cerana cerana against Ascosphaera apis infection[J]. Acta Microbiologica Sinica, 2026 , 66 (5) : 2393 -2403 . DOI: 10.13343/j.cnki.wsxb.20250837
Funding
Less
  • The National Natural Science Foundation of China(32372943)
  • The China National Bee Industry Technology System(CARS-44-KXJ7)
  • The Natural Science Foundation of Fujian Province(2025J01616)
  • The Natural Science Foundation of Fujian Province(2023J01447)
  • The Fujian Agriculture and Forestry University Science and Technology Innovation Special Fund(KFb22060XA)
Appendix
Less
Year 2026 volume 66 Issue 5
PDF
97
42
Cite this Article
BibTeX
Article Info
doi: 10.13343/j.cnki.wsxb.20250837
  • Receive Date：2025-11-07
  • Online Date：2026-05-09
  • Published：2026-05-04
Article Data
Affiliations
History
  • Received：2025-11-07
  • Accepted：2026-01-11
Funding
The National Natural Science Foundation of China(32372943)
The China National Bee Industry Technology System(CARS-44-KXJ7)
The Natural Science Foundation of Fujian Province(2025J01616)
The Natural Science Foundation of Fujian Province(2023J01447)
The Fujian Agriculture and Forestry University Science and Technology Innovation Special Fund(KFb22060XA)
Affiliations
    1.College of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China
    2.National & Local United Engineering Laboratory of Natural Biotoxin, Fuzhou, Fujian, China
    3.Apitherapy Research Institute of Fujian Agriculture and Forestry University, Fuzhou, Fujian, China

Corresponding:

E-mail: CHEN Dafu, ;
FU Zhongmin,
References
Share
https://castjournals.cast.org.cn/joweb/wswxb/EN/10.13343/j.cnki.wsxb.20250837
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House