Home Journals Articles Updates About
CN
image
收藏切换
Isolation and characterization of a lipase- and biosurfactant-producing strain of Stenotrophomonas maltophilia
收藏切换
PDF
Xiaodong TIAN1, Zheng ZHANG1, 2, Qihui MENG1, Jiatong CHEN1, Mengyuan JIA1, Shen WANG1, Chuyun HUANG1, Zongxia CHEN3, Ruofeng HUANG3, Mingguo JIANG1, 2
Acta Microbiologica Sinica | 2026, 66(4) : 1785 - 1801
Less
收藏切换
Acta Microbiologica Sinica | 2026, 66(4): 1785-1801
• Research Article •
Isolation and characterization of a lipase- and biosurfactant-producing strain of Stenotrophomonas maltophilia
Full
Xiaodong TIAN1, Zheng ZHANG1, 2, Qihui MENG1, Jiatong CHEN1, Mengyuan JIA1, Shen WANG1, Chuyun HUANG1, Zongxia CHEN3, Ruofeng HUANG3, Mingguo JIANG1, 2
Affiliations
  • 1.Guangxi Key Laboratory for Polysaccharide Materials and Modification, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning, Guangxi, China
  • 2.Guangxi-Indonesia Joint Laboratory for Microbial Resources and Artificial Intelligence, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning, Guangxi, China
  • 3.Nanning Doing Higher Bio-Tech Co. , Ltd. , Nanning, Guangxi, China
Published: 2026-04-04 doi: 10.13343/j.cnki.wsxb.20251006
Outline
收藏切换

Kitchen waste contains recalcitrant lipids that are prone to rancidification and can cause environmental pollution. Objective To isolate efficient lipase-producing strains from kitchen waste, optimize their enzyme production conditions, and evaluate the lipid-degrading potential of their extracellular products in kitchen waste. Methods Lipase-producing strains were isolated from canteen swill via the neutral red medium and identified based on morphological characteristics and 16S rRNA gene sequences. Lipase production conditions were optimized through single-factor experiments and response surface methodology. The properties of the lipase and the emulsification performance of extracellular polymeric substances (EPS) were analyzed. Results A strain designated C24202, exhibiting strong lipase- and biosurfactant-producing activity, was isolated and identified as Stenotrophomonas maltophilia. The fermentation conditions were optimized as follows: lactose 10.0 g/L, yeast extract 7.5 g/L, emulsified soybean oil 40.0 g/L, FeSO4 8.0 g/L, and incubation at 34 ℃ and initial pH 6.5 for 72 h. Under these conditions, the lipase activity reached (229.64±2.17) U/mL, representing a 2.55-fold increase compared with the pre-optimization level. The lipase exhibited an optimal temperature of 60 ℃ and retained 50.70% of its activity after incubation at 50 ℃ for 6 h, demonstrating good thermal stability. The EPS produced by strain C24202 showed strong emulsifying capacity, with an EI24 value of 46.92%. FTIR analysis suggested that the EPS may be polymeric glycolipopeptide-type biosurfactants. Conclusion Strain C24202 possesses dual capabilities of producing thermostable lipase and biosurfactants, demonstrating promising potential for lipid degradation and the treatment of oil-containing wastewater.

kitchen waste  /  Stenotrophomonas maltophilia  /  thermostable lipase  /  biosurfactant  /  optimization of enzyme production conditions
Xiaodong TIAN, Zheng ZHANG, Qihui MENG, Jiatong CHEN, Mengyuan JIA, Shen WANG, Chuyun HUANG, Zongxia CHEN, Ruofeng HUANG, Mingguo JIANG. Isolation and characterization of a lipase- and biosurfactant-producing strain of Stenotrophomonas maltophilia[J]. Acta Microbiologica Sinica, 2026 , 66 (4) : 1785 -1801 . DOI: 10.13343/j.cnki.wsxb.20251006
Funding
Less
  • National Natural Science Foundation of China(32460009)
  • Science and Technology Backyard in Liangqing, Guangxi Zhuang Autonomous Region(HT-00001499-004)
  • Guangxi Minzu University Graduate Education Innovation Program(gxmzu-chxs2024240)
Appendix
Less
Year 2026 volume 66 Issue 4
PDF
103
46
Cite this Article
BibTeX
Article Info
doi: 10.13343/j.cnki.wsxb.20251006
  • Receive Date：2025-12-30
  • Online Date：2026-04-14
  • Published：2026-04-04
Article Data
Affiliations
History
  • Received：2025-12-30
  • Accepted：2026-02-26
Funding
National Natural Science Foundation of China(32460009)
Science and Technology Backyard in Liangqing, Guangxi Zhuang Autonomous Region(HT-00001499-004)
Guangxi Minzu University Graduate Education Innovation Program(gxmzu-chxs2024240)
Affiliations
    1.Guangxi Key Laboratory for Polysaccharide Materials and Modification, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning, Guangxi, China
    2.Guangxi-Indonesia Joint Laboratory for Microbial Resources and Artificial Intelligence, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning, Guangxi, China
    3.Nanning Doing Higher Bio-Tech Co. , Ltd. , Nanning, Guangxi, China

Corresponding:

References
Share
https://castjournals.cast.org.cn/joweb/wswxb/EN/10.13343/j.cnki.wsxb.20251006
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House