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Discovery and functional characterization of a novel chondroitinase from Streptomyces lividans TK24
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Zhangliang LIU1, 2, 3, Junyue LI2, 3, Xiaofang HUANG1, Xihong HE1, Jinshan LI3, 4, Zhoujie XIE1, Jin’e LI2, 3
Acta Microbiologica Sinica | 2026, 66(4) : 1975 - 1988
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Acta Microbiologica Sinica | 2026, 66(4): 1975-1988
• Research Article •
Discovery and functional characterization of a novel chondroitinase from Streptomyces lividans TK24
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Zhangliang LIU1, 2, 3, Junyue LI2, 3, Xiaofang HUANG1, Xihong HE1, Jinshan LI3, 4, Zhoujie XIE1, Jin’e LI2, 3
Affiliations
  • 1.College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China
  • 2.State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
  • 3.University of Chinese Academy of Sciences, Beijing, China
  • 4.Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China
Published: 2026-04-04 doi: 10.13343/j.cnki.wsxb.20250839
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Objective Chondroitinases are crucial enzymes for the preparation of low-molecular-weight chondroitin sulfate (CS), yet the existing enzymes are insufficient to meet the demands of diverse applications, highlighting the need to discover novel chondroitinases with enhanced properties. Methods A novel chondroitinase belonging to the polysaccharide lyase family 8 (PL8), designated SlChase, was discovered and identified from the model strain Streptomyces lividans TK24. Following heterologous expression in Escherichia coli and the subsequent purification, a soluble and highly active recombinant SlChase was successfully obtained. Results This enzyme exhibited substantial activity within the temperature range of 30-40 ℃ and pH range of 5.5-6.5, and demonstrated excellent long-term stability during storage at 4 ℃. Mg2+ and dithiothreitol (DTT) moderately enhanced its catalytic activity, whereas metal ions including Zn2+ and Fe3+ exerted inhibitory effects on its activity. Notably, SlChase displayed prominent activity towards unsulfated chondroitin (CS-0S), whereas its catalytic activity towards chondroitin sulfate A/C was drastically decreased. Conclusion The discovered SlChase not only expands the diversity of PL8 family enzymes but also affords a novel enzymatic tool for the specific degradation of unsulfated chondroitin, with promising applications in glycoscience research and related biocatalytic processes. Furthermore, this study provides a paradigm for the exploration and utilization of enzymatic resources derived from Streptomyces spp.

chondroitin sulfate  /  Streptomyces lividans  /  chondroitinase
Zhangliang LIU, Junyue LI, Xiaofang HUANG, Xihong HE, Jinshan LI, Zhoujie XIE, Jin’e LI. Discovery and functional characterization of a novel chondroitinase from Streptomyces lividans TK24[J]. Acta Microbiologica Sinica, 2026 , 66 (4) : 1975 -1988 . DOI: 10.13343/j.cnki.wsxb.20250839
Funding
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  • National Key Research and Development Program of China(2021YFC2103200)
  • National Natural Science Foundation of China(32270055)
Appendix
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Year 2026 volume 66 Issue 4
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Article Info
doi: 10.13343/j.cnki.wsxb.20250839
  • Receive Date：2025-11-07
  • Online Date：2026-04-14
  • Published：2026-04-04
Article Data
Affiliations
History
  • Received：2025-11-07
  • Accepted：2025-12-04
Funding
National Key Research and Development Program of China(2021YFC2103200)
National Natural Science Foundation of China(32270055)
Affiliations
    1.College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China
    2.State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    3.University of Chinese Academy of Sciences, Beijing, China
    4.Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China

Corresponding:

E-mail: LI Jin’e,
XIE Zhoujie,
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Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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