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[Objective] To study the mechanism of plant growth-promoting rhizobacterium (PGPR) in the rhizosphere of sea rice and the effects of PGPR on the growth of terrestrial crops under salt stress. [Methods] The salt tolerance, alkali-reducing ability, and plant growth-promoting effect were determined for 15 bacterial strains isolated from the rhizosphere soil of sea rice. Highly active strains were selected for species identification and construction of a consortium. The effect of the consortium on the seed germination of mung bean was verified. [Results] The 15 strains of PGPR were moderately halophilic and strain SL-1 was an extreme halophile among them. Four halophilic strains had alkali tolerance and alkali-degrading effect, among which strain SH-3 had the highest alkali-degrading effect (16.83%). These four strains demonstrated different plant growth-promoting effects. All the strains could produce extracellular polymers (EPS), and strain SH-3 had the highest EPS production (0.47 g/g). The strains were capable of producing indole-3-acetic acid (IAA), with the yields between 0.70 mg/L and 1.48 mg/L. Three highly active strains SL-1, SM-1 and SH-3 belonging to Bacillus and Enterobacter were used to construct a consortium. Seed germination experiments showed that PGPR and the consortium promoted the seed germination of mung bean under salt stress. Moreover, the consortium showcased stronger promoting effect on seed germination than single PGPR. Compared with the control group, the consortium was more effective at the salt concentration of 15 g/L. Specifically, it improved the root length, germination rate, and simplified vitality index, while decreasing the relative salt injure rate from 80.53% to 18.95% and increasing the salt tolerance threshold of mung bean seeds from 10 g/L to 15 g/L. The data indicated that strains of the consortium coordinated to promote the seed germination of mung bean. The correlation analysis showed a strong positive correlation between EPS and IAA, both of which promoted the seed germination, growth, and development of mung bean under salt stress. [Conclusion] Strains SL-1, SM-1 and SH-3 had strong halophilicity, alkali tolerance, and abilities of degrading alkali, producing EPS and IAA, and promoting plant growth. The findings provide a scientific basis for the rational development and utilization of soil microbial resources and the improvement of saline-alkali soil environment.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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