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Protein elicitors HSyp1 and HSyp2 from Saccharothrix yanglingensis Hhs.015 enhance disease resistance of plants
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Hongjia YU1, 2, Jiayang ZHANG1, 2, Pingping WANG3, Chuanbin CUI3, Xia YAN1, 2, *, Lili HUANG2, 4, *
Acta Microbiologica Sinica | 2025, 65(1) : 268 - 282
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Acta Microbiologica Sinica | 2025, 65(1): 268-282
• Research Articles •
Protein elicitors HSyp1 and HSyp2 from Saccharothrix yanglingensis Hhs.015 enhance disease resistance of plants
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Hongjia YU1, 2, Jiayang ZHANG1, 2, Pingping WANG3, Chuanbin CUI3, Xia YAN1, 2, *, Lili HUANG2, 4, *
Affiliations
  • 1 College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China
  • 2 State Key Laboratory for Crop Stress Resistance and High-efficiency Production, Yangling 712100, Shaanxi, China
  • 3 Shaanxi Tobacco Company, China National Tobacco Corporation, Xi'an 710000, Shaanxi, China
  • 4 College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China
Published: 2025-01-04 doi: 10.13343/j.cnki.wsxb.20240471
Outline
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[Objective] Preliminary studies have identified four protein elicitors from the whole genome of Saccharothrix yanglingensis Hhs.015 that can induce a hypersensitive response (HR) in Nicotiana benthamiana. This study investigated the two elicitors capable of triggering a strong HR and their associated immune mechanisms, aiming to provide a basis for probing into the molecular mechanisms by which Hhs.015 enhances the disease resistance of plants. [Methods] The protein elicitors capable of inducing the immune responses of plants were screened based on the HR and reactive oxygen species (ROS) levels. A prokaryotic expression system was used to purify the proteins. Furthermore, we verified the roles of the protein elicitors in inducing plant disease resistance by observing the disease spots, measuring the activities of defense-related enzymes, and analyzing the expression of defense genes. Additionally, we treated Arabidopsis thaliana seedlings with the protein elicitors to evaluate the plant growth-promoting effects of the elicitors. [Results] The protein elicitors HSyp1 and HSyp2 from Hhs.015 that could induce a HR, callose and ROS deposition, and upregulation of defense genes in N. benthamiana, were screened and found to be soluble. HSyp1 and HSyp2 enhanced the resistance of different plants to Sclerotinia sclerotiorum, Phytophthora capsica, and Valsa mali. Furthermore, HSyp1 and HSyp2 promoted the cotyledon and root development of A. thaliana seedlings, and HSyp2 exhibited stronger plant growth-promoting effect than HSyp1. [Conclusion] This study identified two protein elicitors HSyp1 and HSyp2 capable of triggering immune responses, enhance disease resistance, and promoting growth of plants. The findings provide a theoretical basis for the development of new biocontrol agents and offer experimental evidence for their future field application.

prokaryotic expression  /  protein elicitors  /  hypersensitive response  /  disease resistance of plants  /  plant growth-promoting effect
Hongjia YU, Jiayang ZHANG, Pingping WANG, Chuanbin CUI, Xia YAN, Lili HUANG. Protein elicitors HSyp1 and HSyp2 from Saccharothrix yanglingensis Hhs.015 enhance disease resistance of plants[J]. Acta Microbiologica Sinica, 2025 , 65 (1) : 268 -282 . DOI: 10.13343/j.cnki.wsxb.20240471
Funding
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  • National Natural Science Foundation of China(32072477)
  • Science and Technology Major Project of China National Tobacco Corporation(110202101056(LS-16))
  • Science and Technology Project of Shaanxi Branch of China National Tobacco Corporation(KJ-2021-02)
Appendix
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Year 2025 volume 65 Issue 1
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145
64
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Article Info
doi: 10.13343/j.cnki.wsxb.20240471
  • Receive Date：2024-07-30
  • Online Date：2026-03-21
  • Published：2025-01-04
Article Data
Affiliations
History
  • Received：2024-07-30
  • Accepted：2024-10-18
Funding
National Natural Science Foundation of China(32072477)
Science and Technology Major Project of China National Tobacco Corporation(110202101056(LS-16))
Science and Technology Project of Shaanxi Branch of China National Tobacco Corporation(KJ-2021-02)
Affiliations
    1 College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China
    2 State Key Laboratory for Crop Stress Resistance and High-efficiency Production, Yangling 712100, Shaanxi, China
    3 Shaanxi Tobacco Company, China National Tobacco Corporation, Xi'an 710000, Shaanxi, China
    4 College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China

Corresponding:

*YAN Xia, E-mail:
HUANG Lili, E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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