[Objective] This study isolated the dominant indole-3-acetic acid (IAA)-producing bacteria from the rhizosphere soil of Prunus sachalinensis, determined their plant growth-promoting properties, and clarified the growth-promoting effect by inoculation to the seedlings of the common rootstock Gisela 6, aiming to provide theoretical reference and practical approaches for exploring the biological potential of cherry, establish a benign root-microbial interaction relationship, and solve weak root development. [Methods] We used the beef extract peptone medium to isolate bacteria from the rhizosphere soil and selected various specific media to screen the bacteria and determine the IAA-producing, nitrogen-fixing, phosphorus-solubilizing, potassium-solubilizing, and chemotactic abilities of the bacteria. Then, we constructed a phylogenetic tree based on 16S rRNA gene sequences to identify the bacteria. Finally, potted cherry plants were used to explore the plant growth-promoting effects of the strains. [Results] Five IAA-producing strains were screened from the rhizosphere soil of P. sachalinensis, among which strain D46 had the highest IAA yield (53.10 mg/L). D5 was identified as Priestia sp. D27 and D46 were identified as Enterobacter sp. D43 and D79 were identified as Bacillus sp. All the five strains had the ability to fix nitrogen. D27, D46, and D79 had the ability to solubilize phosphorus, and D5 and D43 had the ability to solubilize potassium. Strains D27, D46, and D79 showed strong integrative chemotaxis to sugars, organic acids, and amino acids. Pot experiments showed that the inoculation of strains D27, D43, D46, and D79 significantly increased the root activity. After the inoculation of strain D27, the total root respiration rate increased by 51.40% compared with that in the control group (CK), and the root respiration rates of glycolysis (EMP), tricarboxylic acid cycle (TCA), and pentose phosphate pathway (PPP) also significantly increased. Strain D27 significantly improved the root architecture of cherry seedlings. After inoculation of strain D5, the net photosynthetic rate of leaves significantly increased by 58.82% compared with that in CK, and the inoculation of strain D27 demonstrated the best performance in improving the water use efficiency of leaves. Strains D27, D46, and D79 significantly increased the plant biomass. [Conclusion] There were IAA-producing bacteria in the rhizosphere of cherry, and the five IAA-producing strains isolated had other plant growth-promoting properties. Strains D27, D46, and D79 demonstrated comprehensive plant growth-promoting effects on cherry seedlings. In the future, we can explore the spectra of plants with growth promoted by the strains and the environmental tolerance of the strains, providing a theoretical basis for mining and enriching the strain resources of plant growth-promoting rhizobacteria.