Home Journals Articles Updates About
CN
image
收藏切换
ZntR modulates zinc homeostasis, oxidative stress resistance, and virulence of Vibrio parahaemolyticus
收藏切换
PDF
Mengxian WANG, Yimeng ZHAI, Jun QIU, Zhengzhong XU, Xiang CHEN, Chengkun ZHENG*, Xin'an JIAO*
Acta Microbiologica Sinica | 2024, 64(12) : 4774 - 4788
Less
收藏切换
Acta Microbiologica Sinica | 2024, 64(12): 4774-4788
• Research Articles •
ZntR modulates zinc homeostasis, oxidative stress resistance, and virulence of Vibrio parahaemolyticus
Full
Mengxian WANG, Yimeng ZHAI, Jun QIU, Zhengzhong XU, Xiang CHEN, Chengkun ZHENG*, Xin'an JIAO*
Affiliations
  • College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, Jiangsu, China
Published: 2024-09-26 doi: 10.13343/j.cnki.wsxb.20240274
Outline
收藏切换

[Objective] To explore the regulatory role of the metalloregulator ZntR in metal homeostasis and clarify the effects of ZntR on the oxidative stress resistance and virulence of Vibrio parahemolyticus. [Methods] Growth curve analysis and intracellular metal content quantification were performed to investigate the regulatory effect of ZntR on the metal homeostasis in V. parahaemolyticus. The effects of ZntR on the oxidative stress resistance and virulence of V. parahaemolyticus were explored by the growth curve analysis and the competitive infection assay in the zebrafish model, respectively. The genes regulated by ZntR were identified by RNA sequencing. [Results] The zntR-deleted strain (ΔzntR) exhibited growth defects under zinc, nickel excess and iron restriction conditions, and the growth defects were related to zinc homeostasis disturbance. The overexpression of zntA in ΔzntR promoted the growth under zinc, nickel excess and iron restriction conditions. In the case of zinc excess, ΔzntR demonstrated weakened resistance to H2O2-induced oxidative stress. The virulence of ΔzntR was attenuated in a competitive infection assay in the zebrafish model. RNA sequencing revealed that ZntR regulated the expression of several virulence genes. [Conclusion] ZntR modulates zinc homeostasis and improves oxidative stress resistance and virulence of V. parahaemolyticus.

ZntR  /  Vibrio parahaemolyticus  /  zinc homeostasis  /  oxidative stress resistance  /  virulence
Mengxian WANG, Yimeng ZHAI, Jun QIU, Zhengzhong XU, Xiang CHEN, Chengkun ZHENG, Xin'an JIAO. ZntR modulates zinc homeostasis, oxidative stress resistance, and virulence of Vibrio parahaemolyticus[J]. Acta Microbiologica Sinica, 2024 , 64 (12) : 4774 -4788 . DOI: 10.13343/j.cnki.wsxb.20240274
Funding
Less
  • National Natural Science Foundation of China(31802210)
  • Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
Appendix
Less
Year 2024 volume 64 Issue 12
PDF
98
37
Cite this Article
BibTeX
Article Info
doi: 10.13343/j.cnki.wsxb.20240274
  • Receive Date：2024-04-30
  • Online Date：2026-03-21
  • Published：2024-09-26
Article Data
Affiliations
History
  • Received：2024-04-30
  • Accepted：2024-09-24
Funding
National Natural Science Foundation of China(31802210)
Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
Affiliations
    College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, Jiangsu, China

Corresponding:

*E-mail: ZHENG Chengkun:
E-mail: JIAO Xin'an:
References
Share
https://castjournals.cast.org.cn/joweb/wswxb/EN/10.13343/j.cnki.wsxb.20240274
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House