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Characterization of Streptomyces levis L2 with plant disease-inhibiting and growth-promoting effects
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Jun HUANG1, 2, 3, Ying TANG1, 2, 3, *, Binbin HUANG1, 2, 3, Ye LI1, 2, 3, Cuiyang ZHANG1, 2, 3, Zhaohui GUO1, 2, 3, Qingshu LIU1, 2, 3, *
Acta Microbiologica Sinica | 2024, 64(12) : 4859 - 4868
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Acta Microbiologica Sinica | 2024, 64(12): 4859-4868
• Research Articles •
Characterization of Streptomyces levis L2 with plant disease-inhibiting and growth-promoting effects
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Jun HUANG1, 2, 3, Ying TANG1, 2, 3, *, Binbin HUANG1, 2, 3, Ye LI1, 2, 3, Cuiyang ZHANG1, 2, 3, Zhaohui GUO1, 2, 3, Qingshu LIU1, 2, 3, *
Affiliations
  • 1 Hunan Institute of Microbiology, Changsha 410009, Hunan, China
  • 2 Agricultural Microbiology Application Engineering and Technology Research Center of Hunan Province, Changsha 410009, Hunan, China
  • 3 National Collection of Plant-associated Microbes (Hunan), Changsha 410009, Hunan, China
Published: 2024-09-20 doi: 10.13343/j.cnki.wsxb.20240436
Outline
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[Objective] To study the plant disease-inhibiting and growth-promoting effects and identify the antimicrobial components of Streptomyces levis L2. [Methods] Morphological features and the phylogenetic tree based on the 16S rRNA gene sequences were employed to identify the strain L2 isolated from the rhizosphere of drought-tolerant Echinochloa crusgalli. The antimicrobial components produced by the strain were identified by chromatography and high-resolution mass spectrometry. The whole genome of the strain was sequenced by Illumina in combination with Nanopore. antiSMASH was employed to search for the biosynthetic gene clusters. [Results] Streptomyces levis L2 and its fermentation broth inhibited the growth of Gram-positive bacteria and phytopathogenic fungi, and the strain produced a large transparent zone on the CAS (chrome azurol sulphonate) plate and could produce indole-3-acetic acid (IAA). The antimicrobial components of the strain showed the m/z of 537.102 0 [M+H]+ (calcd for C27H20O12, 537.103 5, 2.2×10–6) and 523.086 3 [M+H]+ (calcd for C26H18O12, 523.087 8, 2.9×10–6), which were consistent with the exact masses of α-rubromycins, β-rubromycins and γ-rubromycins, with the errors less than parts per 5 million. Their HPLC retention time were in agreement with that of standard rubromycins. The whole genome of L2 had a length of 8.8 Mb and carried 32 biosynthetic gene clusters for secondary metabolites including rubromycins. [Conclusion] S. levis L2 exhibited excellent plant disease-inhibiting and growth-promoting properties, thus could be further developed as biocontrol agents. It produces the antimicrobial components rubromycins and its biosynthetic gene clusters contained 6 more modification genes than the previously published gene clusters for rubromycins and several genes with unknown functions.

Streptomyces levis  /  plant growth-promoting bacteria (PGPB)  /  rubromycin  /  disease inhibiting and growth promoting
Jun HUANG, Ying TANG, Binbin HUANG, Ye LI, Cuiyang ZHANG, Zhaohui GUO, Qingshu LIU. Characterization of Streptomyces levis L2 with plant disease-inhibiting and growth-promoting effects[J]. Acta Microbiologica Sinica, 2024 , 64 (12) : 4859 -4868 . DOI: 10.13343/j.cnki.wsxb.20240436
Funding
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  • Natural Science Foundation of Hunan Province(2021JJ40306)
  • National Natural Science Foundation of China(32000047)
  • Changsha Municipal Natural Science Foundation(kq2208130)
  • Science and Technology Innovation Program of Hunan Province Project of "Open Competition Mechanism to Select the Best Candidate"(2021NK1040)
  • Science and Technology Program of China Tobacco Hunan Industrial Co., Ltd.(KY2023YC0009)
Appendix
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Year 2024 volume 64 Issue 12
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93
47
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Article Info
doi: 10.13343/j.cnki.wsxb.20240436
  • Receive Date：2024-07-17
  • Online Date：2026-03-21
  • Published：2024-09-20
Article Data
Affiliations
History
  • Received：2024-07-17
  • Accepted：2024-09-18
Funding
Natural Science Foundation of Hunan Province(2021JJ40306)
National Natural Science Foundation of China(32000047)
Changsha Municipal Natural Science Foundation(kq2208130)
Science and Technology Innovation Program of Hunan Province Project of "Open Competition Mechanism to Select the Best Candidate"(2021NK1040)
Science and Technology Program of China Tobacco Hunan Industrial Co., Ltd.(KY2023YC0009)
Affiliations
    1 Hunan Institute of Microbiology, Changsha 410009, Hunan, China
    2 Agricultural Microbiology Application Engineering and Technology Research Center of Hunan Province, Changsha 410009, Hunan, China
    3 National Collection of Plant-associated Microbes (Hunan), Changsha 410009, Hunan, China

Corresponding:

*E-mail: TANG Ying,
E-mail: LIU Qingshu,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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