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PEDV hijacks DNA damage pathways and manipulates the cell cycle to promote self-replication
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Jinxiang GONG1, 2, Qianxi ZHANG1, Ziyin YANG1, 2, Wenqing WANG1, Kang FENG1, 2, Zhibang ZHANG1, Taotao YANG1, Kai LI1, Zilong SUN2, Xiaoyan ZHANG1, *, Pengcheng LI1, *
Acta Microbiologica Sinica | 2024, 64(12) : 4850 - 4858
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Acta Microbiologica Sinica | 2024, 64(12): 4850-4858
• Research Articles •
PEDV hijacks DNA damage pathways and manipulates the cell cycle to promote self-replication
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Jinxiang GONG1, 2, Qianxi ZHANG1, Ziyin YANG1, 2, Wenqing WANG1, Kang FENG1, 2, Zhibang ZHANG1, Taotao YANG1, Kai LI1, Zilong SUN2, Xiaoyan ZHANG1, *, Pengcheng LI1, *
Affiliations
  • 1 College of Life Science and Resources and Environment, Yichun University, Yichun 336000, Jiangxi, China
  • 2 College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China
Published: 2024-09-03 doi: 10.13343/j.cnki.wsxb.20240421
Outline
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[Objective] To study the effect of DNA damage response (DDR) on the replication of porcine epidemic diarrhea virus (PEDV). [Methods] Specific inhibitors were used to detect whether DDR pathway was involved in PEDV replication. The comet assay was employed to observe the DNA damage caused by PEDV infection in Vero cells. The changes in the expression levels of proteins in the DDR pathway and cell cycle of PEDV-infected Vero cells were determined by Western blotting and flow cytometry, respectively. [Results] The ATM inhibitor KU55933 significantly inhibited the replication of PEDV, with the virus titer decreasing from (5.50±0.25) log10 TCID50/mL to (3.15±0.15) log10 TCID50/mL. PEDV infection caused DNA damage in Vero cells during 12–60 h. ATM, ATR, Chk1, Chk2, and p53 were activated by PEDV infection of Vero cells. Especially, p-Chk2 and p-p53 showcased high expression during virus replication. In addition, PEDV infection led to the stagnation of Vero cells in the S phase. During virus replication, the expression of Cyclin B1 was first downregulated and then upregulated significantly. [Conclusion] PEDV perhaps utilized DNA damage pathway hijacks the ATM-Chk2 to manipulate the cell cycle and promote self-replication. The results provided a basis for elucidating the replication and infection mechanisms of PEDV and developing new potential antiviral targets.

porcine epidemic diarrhea virus  /  DNA damage  /  cell cycle  /  virus replication
Jinxiang GONG, Qianxi ZHANG, Ziyin YANG, Wenqing WANG, Kang FENG, Zhibang ZHANG, Taotao YANG, Kai LI, Zilong SUN, Xiaoyan ZHANG, Pengcheng LI. PEDV hijacks DNA damage pathways and manipulates the cell cycle to promote self-replication[J]. Acta Microbiologica Sinica, 2024 , 64 (12) : 4850 -4858 . DOI: 10.13343/j.cnki.wsxb.20240421
Funding
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  • National Natural Science Foundation of China(32260880)
  • National Natural Science Foundation of China(31960697)
  • Double Thousand Plan of Jiangxi Province(jxsq2019101056)
  • Natural Science Foundation of Jiangxi Province(20202BABL205008)
Appendix
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Year 2024 volume 64 Issue 12
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102
40
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Article Info
doi: 10.13343/j.cnki.wsxb.20240421
  • Receive Date：2024-07-12
  • Online Date：2026-03-21
  • Published：2024-09-03
Article Data
Affiliations
History
  • Received：2024-07-12
  • Accepted：2024-09-02
Funding
National Natural Science Foundation of China(32260880)
National Natural Science Foundation of China(31960697)
Double Thousand Plan of Jiangxi Province(jxsq2019101056)
Natural Science Foundation of Jiangxi Province(20202BABL205008)
Affiliations
    1 College of Life Science and Resources and Environment, Yichun University, Yichun 336000, Jiangxi, China
    2 College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China

Corresponding:

*E-mail: ZHANG Xiaoyan,
E-mail: LI Pengcheng,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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