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Isolation, identification, and genomic analysis of a prodigiosin-producing strain of Serratia marcescens
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Yuxin ZHANG1, Meiyan WANG1, Dianyi LI1, Jun SI3, Xiaoying BIAN4, Guoqing NIU1, *, Rong LIU1, 2, *
Acta Microbiologica Sinica | 2024, 64(11) : 4290 - 4307
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Acta Microbiologica Sinica | 2024, 64(11): 4290-4307
• Research Articles •
Isolation, identification, and genomic analysis of a prodigiosin-producing strain of Serratia marcescens
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Yuxin ZHANG1, Meiyan WANG1, Dianyi LI1, Jun SI3, Xiaoying BIAN4, Guoqing NIU1, *, Rong LIU1, 2, *
Affiliations
  • 1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
  • 2 Chongqing Key Laboratory of Scientific Utilization of Tobacco Resources, China Tobacco Chongqing Industrial Co., Ltd., Chongqing 400060, China
  • 3 Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
  • 4 State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, Shandong, China
Published: 2024-07-06 doi: 10.13343/j.cnki.wsxb.20240303
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[Objective] To select the elite strains among the microorganisms isolated from the rhizosphere of Brassica oleracea var. capitata and evaluate the secondary metabolism potential of the strains, laying a solid foundation for mining the microbial resources in the rhizosphere of this plant. [Methods] The roots of B. oleracea var. capitata were collected from Xiannüshan Town in Wulong District of Chongqing. The conventional methods were then used to isolate microbial strains, and one strain of Serratia marcescens was selected for genome sequencing (PacBio RS Ⅱ and Illumina HiSeq) and bioinformatic analysis. The antiSMASH was used for the detection and comparison of biosynthetic gene clusters (BGCs) encoding secondary metabolites. Red/ET recombination was employed to capture the BGC for prodigiosin. [Results] One prodigiosin-producing strain of S. marcescens was isolated from the rhizosphere of B. oleracea var. capitata. The genome of this strain contained 11 putative BGCs (1–11) for secondary metabolites, of which 9 BGCs displayed low similarities to the BGCs encoding known compounds. This result suggested that the strain had great potential of producing novel secondary metabolites. Prodigiosin was identified by HPLC and high-resolution mass spectrometry (HRMS). Heterologous expression of BGC4 resulted in the production of prodigiosin in Escherichia coli. Four common promoters were used to drive the expression of BGC in E. coli, and the highest prodigiosin production was observed with the rpoS promoter. [Conclusion] A prodigiosin-producing strain of S. marcescens was isolated from the rhizosphere of B. oleracea var. capitata. The BGC for prodigiosin was captured from the chromosome of this strain and expressed successfully in the surrogate host E. coli BAP1.

Brassica oleracea var. capitata  /  Serratia marcescens  /  prodigiosin  /  genomic analysis  /  biosynthetic gene cluster  /  heterologous expression
Yuxin ZHANG, Meiyan WANG, Dianyi LI, Jun SI, Xiaoying BIAN, Guoqing NIU, Rong LIU. Isolation, identification, and genomic analysis of a prodigiosin-producing strain of Serratia marcescens[J]. Acta Microbiologica Sinica, 2024 , 64 (11) : 4290 -4307 . DOI: 10.13343/j.cnki.wsxb.20240303
Funding
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  • National Key Research and Development Program of China(2020YFA0907700)
Appendix
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Year 2024 volume 64 Issue 11
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Article Info
doi: 10.13343/j.cnki.wsxb.20240303
  • Receive Date：2024-05-15
  • Online Date：2026-03-21
  • Published：2024-07-06
Article Data
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History
  • Received：2024-05-15
  • Accepted：2024-07-03
Funding
National Key Research and Development Program of China(2020YFA0907700)
Affiliations
    1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
    2 Chongqing Key Laboratory of Scientific Utilization of Tobacco Resources, China Tobacco Chongqing Industrial Co., Ltd., Chongqing 400060, China
    3 Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
    4 State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, Shandong, China

Corresponding:

*NIU Guoqing, E-mail:
LIU Rong, E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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