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Effects of endoplasmic reticulum stress on the regulation of the lifespan and autophagy of Saccharomyces cerevisiae by PMT1
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Xiaojing CUI1, 2, 3, Di YUAN4, Xiaodi YANG1, 3, Hengheng ZHANG2, Wenbin GUAN1, 3, Junfang WANG5, Xiaofei PANG6, *, Hongjing CUI1, 5, *
Acta Microbiologica Sinica | 2024, 64(11) : 4262 - 4270
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Acta Microbiologica Sinica | 2024, 64(11): 4262-4270
• Research Articles •
Effects of endoplasmic reticulum stress on the regulation of the lifespan and autophagy of Saccharomyces cerevisiae by PMT1
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Xiaojing CUI1, 2, 3, Di YUAN4, Xiaodi YANG1, 3, Hengheng ZHANG2, Wenbin GUAN1, 3, Junfang WANG5, Xiaofei PANG6, *, Hongjing CUI1, 5, *
Affiliations
  • 1 Guangdong Provincial Key Laboratory of Medical Immunology and Molecular Diagnostics, Dongguan 523808, Guangdong, China
  • 2 Department of Laboratory Medicine, the First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan 523808, Guangdong, China
  • 3 School of Medical Technology, Guangdong Medical University, Dongguan 523808, Guangdong, China
  • 4 The Second Clinical Medical School, Guangdong Medical University, Dongguan 523808, Guangdong, China
  • 5 School of Basic Medical Sciences, Guangdong Medical University, Dongguan 523808, Guangdong, China
  • 6 Department of Pediatrics, Shandong Second Provincial General Hospital, Jinan 250022, Shandong, China
Published: 2024-09-14 doi: 10.13343/j.cnki.wsxb.20240286
Outline
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[Objective] To study the effects of endoplasmic reticulum stress on the regulation of the lifespan and autophagy of Saccharomyces cerevisiae by the protein O-mannosyltransferase 1 (PMT1). [Methods] The double deletion strain (pho8Δ60 pmt1Δ) was constructed based on the genetic homologous recombination. The daughter cells produced by the mother cell of the PMT1-deleted yeast strain (pmt1Δ) treated with tunicamycin (inducing endoplasmic reticulum stress) were counted under a light microscope, and the replicative lifespan of the strain was examined. A microplate reader was used to measure the alkaline phosphatase activity of the pho8Δ60 pmt1Δ strain in the SD-N medium (for inducing autophagy). Western blotting was employed to determine the expression level of the autophagy marker Atg8 in the presence of tunicamycin. The transcript levels of autophagy-related genes ATG1 and ATG8 in the pmt1Δ strain treated with tunicamycin were determined by RT-qPCR. [Results] The replicative lifespan of the pmt1Δ strain was shortened by 38.7%, while the alkaline phosphatase activity of pmt1Δ strain was increased compared with those of the wild type in the presence of tunicamycin. The expression levels of GFP-Atg8 fusion protein and free GFP in the pmt1Δ strain were up-regulated with the increase in the concentration of tunicamycin. The transcript levels of ATG1 and ATG8 were up-regulated in the pmt1Δ strain treated with tunicamycin. [Conclusion] Endoplasmic reticulum stress impairs the replicative lifespan and enhances the autophagy of PMT1-deleted yeast cells.

Saccharomyces cerevisiae  /  tunicamycin  /  endoplasmic reticulum stress  /  autophagy  /  PMT1
Xiaojing CUI, Di YUAN, Xiaodi YANG, Hengheng ZHANG, Wenbin GUAN, Junfang WANG, Xiaofei PANG, Hongjing CUI. Effects of endoplasmic reticulum stress on the regulation of the lifespan and autophagy of Saccharomyces cerevisiae by PMT1[J]. Acta Microbiologica Sinica, 2024 , 64 (11) : 4262 -4270 . DOI: 10.13343/j.cnki.wsxb.20240286
Funding
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  • National Natural Science Foundation of China(31701050)
  • Natural Science Foundation of Guangdong Province(2022A1515010816)
  • Guangdong Medical Research Foundation(A2022368)
  • Doctoral Research Start-up Fund Project of Guangdong Medical University(B2017014)
  • Innovation and Entrepreneurship Training Program for Student of Guangdong Medical University(ZCDS002)
Appendix
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Year 2024 volume 64 Issue 11
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100
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Cite this Article
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Article Info
doi: 10.13343/j.cnki.wsxb.20240286
  • Receive Date：2024-05-07
  • Online Date：2026-03-21
  • Published：2024-09-14
Article Data
Affiliations
History
  • Received：2024-05-07
  • Accepted：2024-09-13
Funding
National Natural Science Foundation of China(31701050)
Natural Science Foundation of Guangdong Province(2022A1515010816)
Guangdong Medical Research Foundation(A2022368)
Doctoral Research Start-up Fund Project of Guangdong Medical University(B2017014)
Innovation and Entrepreneurship Training Program for Student of Guangdong Medical University(ZCDS002)
Affiliations
    1 Guangdong Provincial Key Laboratory of Medical Immunology and Molecular Diagnostics, Dongguan 523808, Guangdong, China
    2 Department of Laboratory Medicine, the First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan 523808, Guangdong, China
    3 School of Medical Technology, Guangdong Medical University, Dongguan 523808, Guangdong, China
    4 The Second Clinical Medical School, Guangdong Medical University, Dongguan 523808, Guangdong, China
    5 School of Basic Medical Sciences, Guangdong Medical University, Dongguan 523808, Guangdong, China
    6 Department of Pediatrics, Shandong Second Provincial General Hospital, Jinan 250022, Shandong, China

Corresponding:

*PANG Xiaofei, E-mail:
CUI Hongjing, E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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