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The disulfide bond formation protein DsbA in Listeria monocytogenes regulates cell-to-cell spread
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Zhe WANG1, #, Guo JIANG1, #, Jingjing LI1, Jianfeng WANG2, Lidong XU1, Jiali XU1, Zhanhong FU1, Qian QIN1, Houhui SONG1, Changyong CHENG1, *, Jing XIA1, *
Acta Microbiologica Sinica | 2024, 64(11) : 4308 - 4318
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Acta Microbiologica Sinica | 2024, 64(11): 4308-4318
• Research Articles •
The disulfide bond formation protein DsbA in Listeria monocytogenes regulates cell-to-cell spread
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Zhe WANG1, #, Guo JIANG1, #, Jingjing LI1, Jianfeng WANG2, Lidong XU1, Jiali XU1, Zhanhong FU1, Qian QIN1, Houhui SONG1, Changyong CHENG1, *, Jing XIA1, *
Affiliations
  • 1 Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A & F University, Hangzhou 311300, Zhejiang, China
  • 2 Technical Center of Ningbo Customs, Ningbo Academy of Quarantine & Inspection Science and Technology, Ningbo 315100, Zhejiang, China
Published: 2024-09-04 doi: 10.13343/j.cnki.wsxb.20240304
Outline
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[Objective] In view of the enhanced cell-to-cell spread ability of the dsbA-deleted strain (ΔdsbA) of Listeria monocytogenes, this study aims to elucidate the mechanism that how the disulfide bond formation protein DsbA mediates this biological process. [Methods] The mRNA and protein levels of virulence factors in the wild type and ΔdsbA were compared by RT-qPCR and Western blotting, respectively. The immunofluorescence co-localization analysis method was employed to observe the impact of DsbA deficiency on the actin recruitment by the virulence factor ActA in the cell-to-cell spread of L. monocytogenes (analyzing the length and quantity of the comet tails formed on one side of the bacteria by co-localization of ActA and actin). The presence or absence of interaction between DsbA and ActA was determined by isothermal titration calorimetry (ITC). [Results] Compared with the wild type, ΔdsbA showed no significant changes in the mRNA levels of virulence factors, downregulated protein levels of InlA, InlB, PlcA, and PlcB, and upregulated protein levels of ActA and LLO. In addition, ΔdsbA showed increased number and average length of comet tails, which indicated that the actin recruitment of ΔdsbA was enhanced. The ITC results revealed that DsbA bound to ActA, which gradually showed endothermic reactions, suggesting the presence of interaction between DsbA and ActA. [Conclusion] This study proved for that DsbA attenuated the recruitment ability of actin by regulating virulence proteins, thus affecting the cell-to-cell spread of L. monocytogenes. The findings help to further dissect the virulence regulatory mechanisms of L. monocytogenes during host infection, which is of great importance for controlling the contamination of zoonotic intracellular pathogens threatening public health.

Listeria monocytogenes  /  disulfide bond formation protein DsbA  /  cell-to-cell spread  /  actin recruitment
Zhe WANG, Guo JIANG, Jingjing LI, Jianfeng WANG, Lidong XU, Jiali XU, Zhanhong FU, Qian QIN, Houhui SONG, Changyong CHENG, Jing XIA. The disulfide bond formation protein DsbA in Listeria monocytogenes regulates cell-to-cell spread[J]. Acta Microbiologica Sinica, 2024 , 64 (11) : 4308 -4318 . DOI: 10.13343/j.cnki.wsxb.20240304
Funding
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  • National Key Research and Development Program of China(2023YFD1801800)
  • Natural Science Foundation of Zhejiang Province(LQ22C180001)
  • National College Students' Innovation and Entrepreneurship Training Program(202310341018)
  • Ningbo Natural Science Foundation(2022J200)
Appendix
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Year 2024 volume 64 Issue 11
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Article Info
doi: 10.13343/j.cnki.wsxb.20240304
  • Receive Date：2024-05-16
  • Online Date：2026-03-21
  • Published：2024-09-04
Article Data
Affiliations
History
  • Received：2024-05-16
  • Accepted：2024-09-03
Funding
National Key Research and Development Program of China(2023YFD1801800)
Natural Science Foundation of Zhejiang Province(LQ22C180001)
National College Students' Innovation and Entrepreneurship Training Program(202310341018)
Ningbo Natural Science Foundation(2022J200)
Affiliations
    1 Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A & F University, Hangzhou 311300, Zhejiang, China
    2 Technical Center of Ningbo Customs, Ningbo Academy of Quarantine & Inspection Science and Technology, Ningbo 315100, Zhejiang, China

Corresponding:

*CHENG Changyong, E-mail:
XIA Jing, E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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