PDF
Full
Outline
[Objective] Certain strains of Ligilactobacillus salivarius possess excellent probiotic properties and strong potential for application. This study characterized strain ATCC 11741 in terms of the growth characteristics, stress tolerance, and adhesion capacity, aiming to provide a theoretical basis for the utilization of L. salivarius. [Methods] L. salivarius ATCC 11741's accuracy was verified through morphological observations and 16S rRNA gene sequencing; its growth characteristics were investigated by tracking growth and acid production curves; additionally, a range of tolerance tests, including those with acid, alkali, bile salts, hyperosmolarity and temperature, were conducted to evaluate its tolerance; lastly, self-aggregation and hydrophobicity experiments were used to indirectly determine its adhesion. [Results] The growth curve of L. salivarius ATCC 11741 was formed like a "S". The strain reached its highest acid production between 2–7 h, stabilizing at pH 4.3 after 14 h. After being cultured in the MRS medium at pH 2.0 for 4 h, the strain had a survival rate of 50.48% and grew well in the pH 4.0–11.0 range. Following 2 h of incubation in the MRS medium with 0.10% bile salt, the strain's survival rate was 94.440 0%. In the MRS medium with 6% NaCl, the strain continued to proliferate. The growth of strain ATCC 11741 was significantly affected by temperature, being promoted at 30–42 ℃ and inhibited at 20 ℃ and 50 ℃. At the 5 h mark, the strain's hydrophobicity (measured by the hydrocarbon compound adhesion method) was 44.5% and its self-aggregation rate was 41.4%. [Conclusion] L. salivarius ATCC 11741 is a strain with fast growth, strong tolerance to acids, alkali, and salts, moderate tolerance to bile salt, a wide range of suitable temperatures, and strong adhesion capacity.
PDF
112
51
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
