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napF3 regulates thermophilic mechanism of Thermoanaerobacter tengcongensis
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Ruotong WANG1, Yajuan LIU1, Hanghui ZHENG1, Yijun CHEN2, Xuerui WAN1, Chunlin ZHAO3, Chuan WANG1, *, Yuze YANG2, *
Acta Microbiologica Sinica | 2024, 64(10) : 3825 - 3839
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Acta Microbiologica Sinica | 2024, 64(10): 3825-3839
• Research Articles •
napF3 regulates thermophilic mechanism of Thermoanaerobacter tengcongensis
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Ruotong WANG1, Yajuan LIU1, Hanghui ZHENG1, Yijun CHEN2, Xuerui WAN1, Chunlin ZHAO3, Chuan WANG1, *, Yuze YANG2, *
Affiliations
  • 1 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730030, Gansu, China
  • 2 Beijing Municipal Animal Husbandry Station, Beijing 100101, China
  • 3 Tianshui Normal University, Tianshui 741000, Gansu, China
Published: 2024-07-11 doi: 10.13343/j.cnki.wsxb.20240223
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[Objective] To study the role of napF3 in Thermoanaerobacter tengcongensis at different temperatures. [Methods] We constructed ΔnapF3 from T. tengcongensis by homologous recombination and observed the growth of ΔnapF3 at 50 ℃, 60 ℃, 75 ℃, and 80 ℃. Transcriptome sequencing was employed to identify the differentially expressed genes (DEGs) between ΔnapF3 and the wild type (WT) at 75 ℃. real-time PCR was conducted to measure the transcriptional levels of 13 genes and 3 sRNAs in WT and ΔnapF3 at 50 ℃, 60 ℃, 75 ℃, and 80 ℃. [Results] ΔnapF3 was successfully constructed, and it showcased suspended growth at 50 ℃ and 80 ℃ and slow growth at 60 ℃ and 75 ℃. A total of 899 DEGs between WT and ΔnapF3 at 75 ℃ were identified, including 363 genes with up-regulated expression and 536 genes with down-regulated expression. These DEGs were mainly involved in the biosynthesis of valine, leucine and isoleucine, ABC transporters, two-component system, fatty acid synthesis, thiamine metabolism and other pathways. The transcriptional levels of 13 genes and 3 sRNAs related to the thermophilic mechanism changed under specific temperatures. [Conclusion] napF3 plays a role in the thermophilic adaptation of T. tengcongensis.

Thermoanaerobacter tengcongensis  /  napF3  /  thermophilic adaptation  /  mechanism  /  transcriptome  /  differentially expressed genes
Ruotong WANG, Yajuan LIU, Hanghui ZHENG, Yijun CHEN, Xuerui WAN, Chunlin ZHAO, Chuan WANG, Yuze YANG. napF3 regulates thermophilic mechanism of Thermoanaerobacter tengcongensis[J]. Acta Microbiologica Sinica, 2024 , 64 (10) : 3825 -3839 . DOI: 10.13343/j.cnki.wsxb.20240223
Funding
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  • Youth Mentor Fund of Gansu Agricultural University(GAU-QDFC-2023-04)
  • National Natural Science Foundation of China(31500067)
Appendix
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Year 2024 volume 64 Issue 10
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Article Info
doi: 10.13343/j.cnki.wsxb.20240223
  • Receive Date：2024-04-08
  • Online Date：2026-03-21
  • Published：2024-07-11
Article Data
Affiliations
History
  • Received：2024-04-08
  • Accepted：2024-07-05
Funding
Youth Mentor Fund of Gansu Agricultural University(GAU-QDFC-2023-04)
National Natural Science Foundation of China(31500067)
Affiliations
    1 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730030, Gansu, China
    2 Beijing Municipal Animal Husbandry Station, Beijing 100101, China
    3 Tianshui Normal University, Tianshui 741000, Gansu, China

Corresponding:

*WANG Chuan, E-mail:
YANG Yuze, E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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