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Role of CbrB in the biocontrol strain Pseudomonas protegens Pf-5
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Guangyao RAO1, Cheng WAN2, Hongqiu SHI2, Xiaolin DONG1, *, Daiming ZHA2, *
Acta Microbiologica Sinica | 2024, 64(9) : 3506 - 3520
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Acta Microbiologica Sinica | 2024, 64(9): 3506-3520
• Research Articles •
Role of CbrB in the biocontrol strain Pseudomonas protegens Pf-5
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Guangyao RAO1, Cheng WAN2, Hongqiu SHI2, Xiaolin DONG1, *, Daiming ZHA2, *
Affiliations
  • 1 MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China
  • 2 School of Pharmacy and Life Sciences, Jiujiang University, Jiujiang 332005, Jiangxi, China
Published: 2024-06-05 doi: 10.13343/j.cnki.wsxb.20240168
Outline
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[Objective] To explore the effects of CbrB from the carbon catabolite repression system on the biocontrol performance of Pseudomonas protegens Pf-5. [Methods] The mutant Pf5274 with the markerless deletion of the coding region of cbrB was constructed by a double-crossover recombination event based on pJQ200SK. Moreover, the complementary strain of cbrB and the control strains were constructed by the plasmid complementation method based on pBBR1Am. Lastly, the effects of CbrB on the growth, biofilm formation, motility, antifungal activity, and pyoluteorin synthesis of Pf-5 were analyzed by the measurement of OD600, crystal violet staining, agar plate culture, plate confrontation method, and pltL'-'lacZ fusion report strains, respectively. [Results] CbrB greatly slowed the growth of Pf-5 in the natural medium LB, but greatly sped up the growth of Pf-5 in the basic medium M9-glucose. In addition, CbrB significantly promoted the motility but inhibited the biofilm formation, antifungal activity, and pyoluteorin synthesis of Pf-5. [Conclusion] CbrB plays a role in regulating the growth, biofilm formation, motility, antifungal activity, and pyoluteorin synthesis of P. protegens Pf-5, thus regulating the biocontrol performance of this strain. This study provides a theoretical basis for the biocontrol capabilities of strains by genetic engineering and lays a foundation for probing into the biosynthesis of pyoluteorin.

Pseudomonas protegens  /  CbrB  /  biocontrol  /  pyoluteorin  /  motility  /  biofilm formation
Guangyao RAO, Cheng WAN, Hongqiu SHI, Xiaolin DONG, Daiming ZHA. Role of CbrB in the biocontrol strain Pseudomonas protegens Pf-5[J]. Acta Microbiologica Sinica, 2024 , 64 (9) : 3506 -3520 . DOI: 10.13343/j.cnki.wsxb.20240168
Funding
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  • National Natural Science Foundation of China(31760534)
  • Natural Science Foundation of Jiangxi Province(20202BAB213022)
Appendix
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Year 2024 volume 64 Issue 9
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Article Info
doi: 10.13343/j.cnki.wsxb.20240168
  • Receive Date：2024-03-19
  • Online Date：2026-03-20
  • Published：2024-06-05
Article Data
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History
  • Received：2024-03-19
  • Accepted：2024-06-03
Funding
National Natural Science Foundation of China(31760534)
Natural Science Foundation of Jiangxi Province(20202BAB213022)
Affiliations
    1 MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China
    2 School of Pharmacy and Life Sciences, Jiujiang University, Jiujiang 332005, Jiangxi, China

Corresponding:

*DONG Xiaolin, E-mail:
ZHA Daiming, E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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