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Rapid antimicrobial susceptibility test based on in-situ time-lapsed imaging of microcolonies
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Qianhui GAO1, 2, Yiwen XU2, Hongyi CHEN1, 2, Huijie HU2, Siyu MENG2, Changsong ZHANG3, Ting WANG3, Zhiqiang ZHANG1, 2, *, Yizhi SONG1, 2, *
Acta Microbiologica Sinica | 2024, 64(9) : 3533 - 3546
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Acta Microbiologica Sinica | 2024, 64(9): 3533-3546
• Technology and Method •
Rapid antimicrobial susceptibility test based on in-situ time-lapsed imaging of microcolonies
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Qianhui GAO1, 2, Yiwen XU2, Hongyi CHEN1, 2, Huijie HU2, Siyu MENG2, Changsong ZHANG3, Ting WANG3, Zhiqiang ZHANG1, 2, *, Yizhi SONG1, 2, *
Affiliations
  • 1 School of Biomedical Engineering (Suzhou), University of Science and Technology of China, Hefei 230026, Anhui, China
  • 2 Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, Jiangsu, China
  • 3 The Affiliated Suzhou Hospital of Nanjing University Medical School (Suzhou Science and Technology Town Hospital), Suzhou 215163, Jiangsu, China
Published: 2024-05-30 doi: 10.13343/j.cnki.wsxb.20240113
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[Objective] Over 7 million people die from bacterial infections every year due to the inappropriate or untimely use of antibiotics. Antimicrobial susceptibility test (AST) is a routine method guiding antibiotic therapy in clinical practice, while the existing methods have limitations as they require at least 16–20 h to get the results. We aimed to develop a novel rapid AST method based on bacterial morphology analysis, which can improve the diagnosis efficiency and provide timely treatment for the patients with bacterial infections, especially for urgent cases. [Methods] We developed an in-situ time-lapsed imaging (ISM-TLI) technique for microcolonies by combining bacterial incubation, in vitro antibiotic treatment, microscopic imaging, and image processing algorithm. The combination of the imaging device with the algorithm enabled the tracing of the morphological changes of colonies treated with different concentrations of antibiotics in-situ and the rapid obtainment of AST results. [Results] This technique worked effectively for both Gram-positive and Gram-negative bacteria and could determine the minimum inhibitory concentration (MIC) within 2 h, with the results in agreement with those obtained with the clinical gold standard method. [Conclusion] Our rapid AST technique significantly reduced the turn-around-time for obtaining the AST profile and had great potential for AST of a wide range of other strains.

Hough transform  /  bacterial morphology  /  image recognition  /  rapid antimicrobial susceptibility test  /  in-situ incubation
Qianhui GAO, Yiwen XU, Hongyi CHEN, Huijie HU, Siyu MENG, Changsong ZHANG, Ting WANG, Zhiqiang ZHANG, Yizhi SONG. Rapid antimicrobial susceptibility test based on in-situ time-lapsed imaging of microcolonies[J]. Acta Microbiologica Sinica, 2024 , 64 (9) : 3533 -3546 . DOI: 10.13343/j.cnki.wsxb.20240113
Funding
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  • National Key Research and Development Program of China(2022YFC2403300)
  • National Natural Science Foundation of China(32170173)
Appendix
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Year 2024 volume 64 Issue 9
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84
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Article Info
doi: 10.13343/j.cnki.wsxb.20240113
  • Receive Date：2024-02-23
  • Online Date：2026-03-20
  • Published：2024-05-30
Article Data
Affiliations
History
  • Received：2024-02-23
  • Accepted：2024-05-13
Funding
National Key Research and Development Program of China(2022YFC2403300)
National Natural Science Foundation of China(32170173)
Affiliations
    1 School of Biomedical Engineering (Suzhou), University of Science and Technology of China, Hefei 230026, Anhui, China
    2 Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, Jiangsu, China
    3 The Affiliated Suzhou Hospital of Nanjing University Medical School (Suzhou Science and Technology Town Hospital), Suzhou 215163, Jiangsu, China

Corresponding:

*ZHANG Zhiqiang, E-mail:
SONG Yizhi, E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
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Percentage of total
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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