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Effects of adding garlic juice on pickled Chinese cabbage
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Quanmin SUN1, 2, 3, 4, #, Lu ZHAO1, 2, 3, 4, #, Wenli CHEN1, 2, 3, 4, Jiaming WU1, 2, 3, 4, Naiyu CHI1, 2, 3, 4, Xiaohui WANG1, 2, 3, 4, *, Qingfang ZHANG1, 2, 3, 4, *
Acta Microbiologica Sinica | 2024, 64(9) : 3345 - 3365
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Acta Microbiologica Sinica | 2024, 64(9): 3345-3365
Research Articles
Effects of adding garlic juice on pickled Chinese cabbage
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Quanmin SUN1, 2, 3, 4, #, Lu ZHAO1, 2, 3, 4, #, Wenli CHEN1, 2, 3, 4, Jiaming WU1, 2, 3, 4, Naiyu CHI1, 2, 3, 4, Xiaohui WANG1, 2, 3, 4, *, Qingfang ZHANG1, 2, 3, 4, *
Affiliations
  • 1 College of Life and Health, Dalian University, Dalian 116622, Liaoning, China
  • 2 Liaoning Marine Microbial Engineering and Technology Center, Dalian 116622, Liaoning, China
  • 3 Key Laboratory of Marine Microbial Engineering in Dalian City, Dalian 116622, Liaoning, China
  • 4 Key Laboratory of Synthetic Biology in Dalian City, Dalian 116622, Liaoning, China
Published: 2024-05-28 doi: 10.13343/j.cnki.wsxb.20240123
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[Objective] To study the effects of adding different concentrations of garlic juice on the fermentation of pickled Chinese cabbage. [Methods] The physicochemical indicators and OD600 during the fermentation process were measured, and the effects of garlic juice concentration on the richness, community structure, and abundance of bacteria during the fermentation were studied with natural fermentation as the control. The 16S rRNA gene high-throughput sequencing was carried out in the late fermentation stage for the pickled Chinese cabbage samples with the addition of garlic juice at different concentrations. Fuzzy mathematics-based evaluation was conducted for the sensory quality of garlic-flavored samples to select the lactic acid bacterial strains with excellent fermentation performance. [Results] Different concentrations of garlic juice had minor effects on the pH and OD600 but significantly reduced the peak value of nitrite. Adding 0.2%–0.4% garlic juice was beneficial for the growth of Lactiplantibacillus, and the species richness of the product fermented with the addition of garlic juice was higher than that of the naturally fermented product. The fermentation liquid with the addition of 0.3% garlic juice had the highest species richness, with much higher number of genera than other concentration groups. The product from fermentation with 0.3% garlic juice showcased the best quality, presenting better sensory quality and appearance. The product with high species richness and a large number of genera had good sensory quality. A strain of lactic acid bacteria with excellent fermentation performance was screened out and identified as Lactiplantibacillus pentosus, which produced the most acids during fermentation, had the highest nitrite degradation rate, and exhibited tolerance to garlic. [Conclusion] This study provides a strain for the development of lactic acid bacteria fermentation agents and lays a theoretical foundation for the industrialization of garlic-flavored pickled Chinese cabbage.

garlic juice  /  pickled Chinese cabbage  /  microbial community structure  /  fuzzy mathematics  /  lactic acid bacteria
Quanmin SUN, Lu ZHAO, Wenli CHEN, Jiaming WU, Naiyu CHI, Xiaohui WANG, Qingfang ZHANG. Effects of adding garlic juice on pickled Chinese cabbage[J]. Acta Microbiologica Sinica, 2024 , 64 (9) : 3345 -3365 . DOI: 10.13343/j.cnki.wsxb.20240123
  • Central Government's Guidance Fund for Local Scientific and Technological Development Proiects(110013)
  • National Natural Science Foundation of China(31500039)
  • Dalian City Youth Science and Technology Star(2017RQ155)
  • National Key Research and Development Program of China(2022YFC2805105)
Year 2024 volume 64 Issue 9
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Article Info
doi: 10.13343/j.cnki.wsxb.20240123
  • Receive Date:2024-02-28
  • Online Date:2026-03-20
  • Published:2024-05-28
Article Data
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History
  • Received:2024-02-28
  • Accepted:2024-05-23
Funding
Central Government's Guidance Fund for Local Scientific and Technological Development Proiects(110013)
National Natural Science Foundation of China(31500039)
Dalian City Youth Science and Technology Star(2017RQ155)
National Key Research and Development Program of China(2022YFC2805105)
Affiliations
    1 College of Life and Health, Dalian University, Dalian 116622, Liaoning, China
    2 Liaoning Marine Microbial Engineering and Technology Center, Dalian 116622, Liaoning, China
    3 Key Laboratory of Marine Microbial Engineering in Dalian City, Dalian 116622, Liaoning, China
    4 Key Laboratory of Synthetic Biology in Dalian City, Dalian 116622, Liaoning, China

Corresponding:

*E-mail: WANG Xiaohui,
E-mail: ZHANG Qingfang,
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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