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Biological characterization and genome sequence of a bacteriophage strain Bac-S infecting Bacillus subtilis
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Xinzhuo ZHAO1, 3, Xinmiao WANG1, 3, Liyan WANG1, 3, Nan WU1, Ximei LI1, Zhenhua YU2, Ruiyong JING1, 3, *, Junjie LIU2, *
Acta Microbiologica Sinica | 2024, 64(9) : 3379 - 3392
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Acta Microbiologica Sinica | 2024, 64(9): 3379-3392
• Research Articles •
Biological characterization and genome sequence of a bacteriophage strain Bac-S infecting Bacillus subtilis
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Xinzhuo ZHAO1, 3, Xinmiao WANG1, 3, Liyan WANG1, 3, Nan WU1, Ximei LI1, Zhenhua YU2, Ruiyong JING1, 3, *, Junjie LIU2, *
Affiliations
  • 1 Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Argo-waste in Cold Region, College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China
  • 2 Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, Heilongjiang, China
  • 3 Key Laboratory of Low-carbon Green Agriculture in Northeast Plain, Ministry of Agriculture and Rural Affairs, Daqing 163319, Heilongjiang, China
Published: 2024-05-08 doi: 10.13343/j.cnki.wsxb.20240130
Outline
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[Objective] The application of Bacillus subtilis in soil can promote plant growth, while few studies have reported the bacteriophages infecting B. subtilis. Therefore, the isolation, biological characterization, and genome sequencing of bacteriophages infecting B. subtilis from soil will contribute to the application of B. subtilis and enrich the biological information of bacteriophages infecting B. subtilis. [Methods] B. subtilis SM13 was used as the host to isolate a bacteriophage strain Bac-S from soil. Biological characterization, whole genome sequencing, gene function annotation, and phylogenetic analysis were performed for this bacteriophage strain. [Results] Transmission electron microscopy showed that Bac-S had a head with the diameter of about 43 nm and a tail too short to be measured. The optimal multiplicity of infection of Bac-S was 0.1. The one-step growth curve showed that Bac-S had an incubation period of about 10 min and a burst size of 30 PFU/cell. Bac-S had a wide host spectrum and can infect hosts of different genera. The sequencing results showed that the genome of Bac-S was 150 019 bp, with the G+C content of 42.6% and 237 open reading frames (ORFs). The BLASTn comparison with the sequences in the NCBI database showed that Bac-S was similar to other bacteriophages infecting B. subtilis. [Conclusion] A bacteriophage strain infecting B. subtilis with a short incubation period, a wide host spectrum, tolerance to high temperature, and intolerance to UV light was isolated from soil. The biological and genetic characterization of this bacteriophage enriches our knowledge about the bacteriophages infecting B. subtilis.

Bacillus subtilis  /  bacteriophage  /  biological characteristics  /  genome
Xinzhuo ZHAO, Xinmiao WANG, Liyan WANG, Nan WU, Ximei LI, Zhenhua YU, Ruiyong JING, Junjie LIU. Biological characterization and genome sequence of a bacteriophage strain Bac-S infecting Bacillus subtilis[J]. Acta Microbiologica Sinica, 2024 , 64 (9) : 3379 -3392 . DOI: 10.13343/j.cnki.wsxb.20240130
Funding
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  • National Key Research and Development Program of China(2021YFD1500400)
  • National Natural Science Foundation of China(31870477)
  • National Natural Science Foundation of China(31300425)
  • Natural Science Foundation of Heilongjiang Province(C2017045)
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Year 2024 volume 64 Issue 9
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Article Info
doi: 10.13343/j.cnki.wsxb.20240130
  • Receive Date：2024-03-02
  • Online Date：2026-03-20
  • Published：2024-05-08
Article Data
Affiliations
History
  • Received：2024-03-02
  • Accepted：2024-04-30
Funding
National Key Research and Development Program of China(2021YFD1500400)
National Natural Science Foundation of China(31870477)
National Natural Science Foundation of China(31300425)
Natural Science Foundation of Heilongjiang Province(C2017045)
Affiliations
    1 Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Argo-waste in Cold Region, College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China
    2 Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, Heilongjiang, China
    3 Key Laboratory of Low-carbon Green Agriculture in Northeast Plain, Ministry of Agriculture and Rural Affairs, Daqing 163319, Heilongjiang, China

Corresponding:

*E-mail: JING Ruiyong,
E-mail: LIU Junjie,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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