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Adjuvant activity of outer membrane vesicles from Salmonella minnesota Re595 with lipopolysaccharides modified for attenuating toxicity
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Jiawen ZHANG1, 2, Wenzhu YIN1, Haiyan WANG1, Jinqiu ZHANG1, Bihua DENG1, Yu LU1, 3, Mingxu ZHOU1, 3, *, Fang MA1, 3, *
Acta Microbiologica Sinica | 2024, 64(9) : 3269 - 3281
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Acta Microbiologica Sinica | 2024, 64(9): 3269-3281
• Research Articles •
Adjuvant activity of outer membrane vesicles from Salmonella minnesota Re595 with lipopolysaccharides modified for attenuating toxicity
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Jiawen ZHANG1, 2, Wenzhu YIN1, Haiyan WANG1, Jinqiu ZHANG1, Bihua DENG1, Yu LU1, 3, Mingxu ZHOU1, 3, *, Fang MA1, 3, *
Affiliations
  • 1 National Research Center of Engineering and Technology for Veterinary Biologicals, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu, China
  • 2 College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
  • 3 GuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou 225300, Jiangsu, China
Published: 2024-05-10 doi: 10.13343/j.cnki.wsxb.20240102
Outline
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[Objective] Outer membrane vesicles (OMVs) are spherical bilayer membrane structures secreted by Gram-negative and some Gram-positive bacteria. OMVs contain abundant surface antigens and are of great research significance in vaccine development. However, the presence of lipopolysaccharides (LPS), which is the primary component of OMVs, arouses safety concern. Therefore, genetically modifying bacterial LPS to produce safe and efficient OMVs is a viable approach to enhance the production and application of OMVs. [Methods] We modified Salmonella minnesota Re595 with O antigen and most core antigen deletions by deleting the acyl chain coding gene msbB and inserting the phosphatase coding gene lpxE from Francisella novicida to reduce acyl chains and phosphate groups on lipid A, thus obtaining less toxic LPS. LPS and OMVs were extracted from the starting strain and modified strain, and their pro-inflammatory activities were compared between the two strains. In addition, inactivated foot-and-mouth disease virus vaccines were prepared with OMVs to assess the immune adjuvant activity of OMVs. [Results] The modification of LPS reduced the endotoxin activity and pro-inflammatory responses while significantly increasing the immune adjuvant activity of OMVs. [Conclusion] This study demonstrates that the modification of LPS can attenuate the toxicity and enhance the immune adjuvant activity of bacterial OMVs. These findings provide a theoretical foundation for utilizing OMVs as immune adjuvants in the future.

outer membrane vesicles  /  immune adjuvant  /  lipopolysaccharide  /  endotoxin activity  /  gene editing
Jiawen ZHANG, Wenzhu YIN, Haiyan WANG, Jinqiu ZHANG, Bihua DENG, Yu LU, Mingxu ZHOU, Fang MA. Adjuvant activity of outer membrane vesicles from Salmonella minnesota Re595 with lipopolysaccharides modified for attenuating toxicity[J]. Acta Microbiologica Sinica, 2024 , 64 (9) : 3269 -3281 . DOI: 10.13343/j.cnki.wsxb.20240102
Funding
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  • National Natural Science Foundation of China(31902243)
  • Agriculture Science and Technology Innovation Fund of Jiangsu Province(CX(22)3030)
Appendix
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Year 2024 volume 64 Issue 9
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93
40
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Article Info
doi: 10.13343/j.cnki.wsxb.20240102
  • Receive Date：2024-02-15
  • Online Date：2026-03-20
  • Published：2024-05-10
Article Data
Affiliations
History
  • Received：2024-02-15
  • Accepted：2024-04-19
Funding
National Natural Science Foundation of China(31902243)
Agriculture Science and Technology Innovation Fund of Jiangsu Province(CX(22)3030)
Affiliations
    1 National Research Center of Engineering and Technology for Veterinary Biologicals, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu, China
    2 College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
    3 GuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou 225300, Jiangsu, China

Corresponding:

*E-mail: ZHOU Mingxu,
E-mail: MA Fang,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
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Genus		 种数
Number of
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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