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Multiomics analysis of Escherichia coli Nissle 1917 and functional validation of microcin
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Jiabing TANG, Ying ZHANG, Jiawei YE, Xian ZHANG, Zhiming RAO, Meijuan XU*
Acta Microbiologica Sinica | 2024, 64(9) : 3238 - 3252
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Acta Microbiologica Sinica | 2024, 64(9): 3238-3252
• Research Articles •
Multiomics analysis of Escherichia coli Nissle 1917 and functional validation of microcin
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Jiabing TANG, Ying ZHANG, Jiawei YE, Xian ZHANG, Zhiming RAO, Meijuan XU*
Affiliations
  • Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China
Published: 2024-04-03 doi: 10.13343/j.cnki.wsxb.20240097
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[Objective] To compare the metabolism and transcription between the probiotic Escherichia coli Nissle 1917 (EcN) and the model strains, thus providing a reference for the engineering and promoting the application of the food-safe strain EcN. [Methods] The genome and transcriptome were compared between EcN and model strains BL21(DE3) and W3110 by software, and plasmids were constructed to verify the differences. EcN-derived microcin was expressed in BL21(DE3) and the antibacterial effect of microcin was verified. [Results] A total of 904 differentially coding genes were identified. The differences in carbon source absorption and utilization of different strains were verified by experiments with different carbon sources as substrates. The expression of the promoter Pflic confirmed the differences in transcription among different strains. The recombinant strain of microcin showed an increase of 30.3% in the inhibition rate after 12 h of culture. [Conclusion] This study clarifies the metabolic characteristics of EcN and confirms the differences in transcription between EcN and model strains. Moreover, this study provides ideas for the development of microcin as a narrow-spectrum therapeutic drug to inhibit intestinal pathogens and reduce intestinal bacterial blooms.

Escherichia coli  /  Nissle 1917  /  multiomics analysis  /  microcin
Jiabing TANG, Ying ZHANG, Jiawei YE, Xian ZHANG, Zhiming RAO, Meijuan XU. Multiomics analysis of Escherichia coli Nissle 1917 and functional validation of microcin[J]. Acta Microbiologica Sinica, 2024 , 64 (9) : 3238 -3252 . DOI: 10.13343/j.cnki.wsxb.20240097
Funding
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  • National Natural Science Foundation of China(32270036)
  • National Natural Science Foundation of China(32070035)
  • National Key Research and Development Program of China(2023YFD1300700)
  • Fundamental Research Funds for the Central Universities(JUSRP221012)
  • Fundamental Research Funds for the Central Universities(JUSRP622022)
  • Open Project of the Key Laboratory of Industrial Biotechnology of the Ministry of Education(KLIB-KF202305)
Appendix
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Year 2024 volume 64 Issue 9
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135
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Article Info
doi: 10.13343/j.cnki.wsxb.20240097
  • Receive Date：2024-02-06
  • Online Date：2026-03-20
  • Published：2024-04-03
Article Data
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History
  • Received：2024-02-06
  • Accepted：2024-03-29
Funding
National Natural Science Foundation of China(32270036)
National Natural Science Foundation of China(32070035)
National Key Research and Development Program of China(2023YFD1300700)
Fundamental Research Funds for the Central Universities(JUSRP221012)
Fundamental Research Funds for the Central Universities(JUSRP622022)
Open Project of the Key Laboratory of Industrial Biotechnology of the Ministry of Education(KLIB-KF202305)
Affiliations
    Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China

Corresponding:

*XU Meijuan, E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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