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Culture medium optimization of fastidious Parvimonas micra
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Hui LING1, 2, #, Yuxiao CHANG2, #, Dong LI1, 2, Likun WANG2, Huan ZHANG2, Xiaoming QIN2, Yafang TAN2, *, Yujing BI1, 2, *
Acta Microbiologica Sinica | 2024, 64(9) : 3547 - 3558
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Acta Microbiologica Sinica | 2024, 64(9): 3547-3558
• Technology and Method •
Culture medium optimization of fastidious Parvimonas micra
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Hui LING1, 2, #, Yuxiao CHANG2, #, Dong LI1, 2, Likun WANG2, Huan ZHANG2, Xiaoming QIN2, Yafang TAN2, *, Yujing BI1, 2, *
Affiliations
  • 1 School of Basic Medical Sciences, Anhui Medical University, Hefei 230000, Anhui, China
  • 2 State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China
Published: 2024-05-06 doi: 10.13343/j.cnki.wsxb.20240114
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[Objective] To improve the culture medium components of Parvimonas micra, increase the number of live cells, and develop a demonstration method for culturing fastidious bacteria. [Methods] Biochemical analysis was conducted on a strain of P. micra to screen the potential substrates that could promote bacterial growth. A single-factor experiment was carried out for each substrate with three concentrations. The substrate with a significant bacterial enrichment effect was further optimized for concentration, and thus a new culture medium was obtained. [Results] The single-factor experiment results showed that the substrates with significant bacterial enrichment effects included L-serine, L-threonine, and glycyl-L-glutamine. In the medium with the addition of 4.8 g/L L-serine, the live cell count of P. micra reached 3.6×108 CFU/mL, representing a 4.2-fold increase compared with that in the basic medium with tryptone soya broth (TSB) and fetal bovine serum (FBS). Furthermore, the improved culture medium was applied to the culture of another P. micra strain, demonstrating a significant growth-promoting effect. [Conclusion] This study proves that using biochemical identification plates to screen medium supplements is a fast and efficient method, providing a reference for the enlargement culture of fastidious bacteria.

Parvimonas micra  /  fastidious bacterium  /  medium optimization
Hui LING, Yuxiao CHANG, Dong LI, Likun WANG, Huan ZHANG, Xiaoming QIN, Yafang TAN, Yujing BI. Culture medium optimization of fastidious Parvimonas micra[J]. Acta Microbiologica Sinica, 2024 , 64 (9) : 3547 -3558 . DOI: 10.13343/j.cnki.wsxb.20240114
Funding
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  • National Key Research and Development Program of China(2021YFC2301000)
Appendix
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Year 2024 volume 64 Issue 9
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Article Info
doi: 10.13343/j.cnki.wsxb.20240114
  • Receive Date：2024-02-23
  • Online Date：2026-03-20
  • Published：2024-05-06
Article Data
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History
  • Received：2024-02-23
  • Accepted：2024-04-28
Funding
National Key Research and Development Program of China(2021YFC2301000)
Affiliations
    1 School of Basic Medical Sciences, Anhui Medical University, Hefei 230000, Anhui, China
    2 State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China

Corresponding:

*TAN Yafang, E-mail:
BI Yujing, E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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