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Origin of CRISPR-Cas: progress in research and applications of TnpB and IscB
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Guangliang SHI1, 2, Wei LI1, *, Hua XIANG2, Luyao GONG2, *
Acta Microbiologica Sinica | 2024, 64(9) : 3091 - 3104
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Acta Microbiologica Sinica | 2024, 64(9): 3091-3104
• Reviews •
Origin of CRISPR-Cas: progress in research and applications of TnpB and IscB
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Guangliang SHI1, 2, Wei LI1, *, Hua XIANG2, Luyao GONG2, *
Affiliations
  • 1 College of Life Science, Sichuan Normal University, Chengdu 610101, Sichuan, China
  • 2 State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Published: 2024-06-03 doi: 10.13343/j.cnki.wsxb.20240099
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CRISPR-Cas is a defense system ubiquitous in bacteria and archaea. It has been successfully applied in genome editing in a variety of organisms. At present, CRISPR-Cas9 and CRISPR-Cas12a are the most widely used genome editing tools. However, the large protein sizes of Cas9 and Cas12a (more than 1 000 amino acids (aa)) hinder their delivery. TnpB and IscB (about 400 aa) encoded by the transposon family are considered ancestors of Cas12 and Cas9, respectively, whereas their functions are revealed just recently. They are named as obligate mobile element-guided activity (OMEGA), with the associated RNA named ωRNA. Since then, the OMEGA system has become one of the research hotspots in genome editing. OMEGA systems are diverse, with wide distribution in all the three domains of life. The in-depth research on the OMEGA system will aid in the development of new genome editing tools that are streamlined, efficient, and safe. Here, we reviewed the discovery history, structural characteristics, mechanisms of cleavage, and genome editing applications of OMEGA systems, aiming to lay a foundation for the development and optimization of genome editing tools.

CRISPR  /  IscB  /  TnpB  /  obligate mobile element-guided activity (OMEGA)  /  Fanzor  /  genome editing
Guangliang SHI, Wei LI, Hua XIANG, Luyao GONG. Origin of CRISPR-Cas: progress in research and applications of TnpB and IscB[J]. Acta Microbiologica Sinica, 2024 , 64 (9) : 3091 -3104 . DOI: 10.13343/j.cnki.wsxb.20240099
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  • National Natural Science Foundation of China(32230061)
Appendix
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Year 2024 volume 64 Issue 9
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Article Info
doi: 10.13343/j.cnki.wsxb.20240099
  • Receive Date：2024-02-07
  • Online Date：2026-03-20
  • Published：2024-06-03
Article Data
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History
  • Received：2024-02-07
  • Accepted：2024-05-31
Funding
National Natural Science Foundation of China(32230061)
Affiliations
    1 College of Life Science, Sichuan Normal University, Chengdu 610101, Sichuan, China
    2 State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

Corresponding:

*E-mail: LI Wei,
E-mail: GONG Luyao,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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