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The glucoside transporter Lmo0738 contributes to virulence of Listeria monocytogenes
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Yunqin SHEN1, #, Jianfeng WANG2, #, Yu YING1, Yanlan WENG1, Yun HAO1, Liying HUANG1, Yue HAN1, Jing SUN1, Xiuling ZHAO2, Houhui SONG1, Jiali XU1, *, Changyong CHENG1, *
Acta Microbiologica Sinica | 2024, 64(8) : 2671 - 2683
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Acta Microbiologica Sinica | 2024, 64(8): 2671-2683
• Research Articles •
The glucoside transporter Lmo0738 contributes to virulence of Listeria monocytogenes
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Yunqin SHEN1, #, Jianfeng WANG2, #, Yu YING1, Yanlan WENG1, Yun HAO1, Liying HUANG1, Yue HAN1, Jing SUN1, Xiuling ZHAO2, Houhui SONG1, Jiali XU1, *, Changyong CHENG1, *
Affiliations
  • 1 Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A & F University, Hangzhou 311300, Zhejiang, China
  • 2 Technical Center of Ningbo Customs, Ningbo Academy of Quarantine & Inspection Science and Technology, Ningbo 315100, Zhejiang, China
Published: 2024-05-07 doi: 10.13343/j.cnki.wsxb.20240010
Outline
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[Objective] To investigate the biological role played by the glucoside transporter Lmo0738 in the virulence of Listeria monocytogenes. [Methods] The lmo0738-deleted mutant (Δlmo0738) and complementation mutant (CΔlmo0738) were constructed by homologous recombination. The growth, hemolytic activity, cellular adhesion and invasion, and intracellular migration of the wild type strain and the mutants were assessed by the growth curves, sheep red blood cell hemolysis assay, infection of human epithelial cells (Caco-2), and infection of mouse fibroblastic cells (L929), respectively. Additionally, the mRNA and protein levels of the virulence factor listeriolysin O (LLO) were determined by real-time quantitative reverse transcription PCR (RT-qPCR) and Western blotting, respectively. [Results] The L. monocytogenes strain with the deletion of lmo0738 demonstrated weakened growth and diminished hemolytic activity. Notably, Δlmo0738 exhibited significantly reduced cell adhesion, invasion, and intracellular migration compared with the wild type strain. In addition, the mRNA and protein levels of LLO were significantly down-regulated in Δlmo0738. [Conclusion] This study provides the evidence that the absence of lmo0738 attenuates the virulence of L. monocytogenes, laying a crucial foundation to illustrate the mechanism of the phosphotransferase system (PTS) in regulating the sugar catabolism and the infection mechanism of major food-borne pathogens including L. monocytogenes.

Listeria monocytogenes  /  glucoside transporter  /  virulence
Yunqin SHEN, Jianfeng WANG, Yu YING, Yanlan WENG, Yun HAO, Liying HUANG, Yue HAN, Jing SUN, Xiuling ZHAO, Houhui SONG, Jiali XU, Changyong CHENG. The glucoside transporter Lmo0738 contributes to virulence of Listeria monocytogenes[J]. Acta Microbiologica Sinica, 2024 , 64 (8) : 2671 -2683 . DOI: 10.13343/j.cnki.wsxb.20240010
Funding
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  • National Key Research and Development Program of China(2023YFD1801800)
  • National Natural Science Foundation of China(32172849)
  • National Natural Science Foundation of China(32302961)
  • Natural Science Foundation of Zhejiang Province(LY23C180002)
  • Ningbo Natural Science Foundation(2022J200)
Appendix
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Year 2024 volume 64 Issue 8
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Article Info
doi: 10.13343/j.cnki.wsxb.20240010
  • Receive Date：2024-01-05
  • Online Date：2026-03-19
  • Published：2024-05-07
Article Data
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History
  • Received：2024-01-05
  • Accepted：2024-04-28
Funding
National Key Research and Development Program of China(2023YFD1801800)
National Natural Science Foundation of China(32172849)
National Natural Science Foundation of China(32302961)
Natural Science Foundation of Zhejiang Province(LY23C180002)
Ningbo Natural Science Foundation(2022J200)
Affiliations
    1 Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A & F University, Hangzhou 311300, Zhejiang, China
    2 Technical Center of Ningbo Customs, Ningbo Academy of Quarantine & Inspection Science and Technology, Ningbo 315100, Zhejiang, China

Corresponding:

*CHENG Changyong, E-mail:
XU Jiali, E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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