Home Journals Articles Updates About
CN
image
收藏切换
A CRISPR/Cas13a-based nucleic acid detection method for Marburg virus
收藏切换
PDF
Kuocheng YAN1, Hao LI1, Xuanyang BAI2, Yao HAN1, Dawei SHI3, Leili JIA2, *, Yansong SUN1, *
Acta Microbiologica Sinica | 2024, 64(8) : 3073 - 3085
Less
收藏切换
Acta Microbiologica Sinica | 2024, 64(8): 3073-3085
• Technology and Method •
A CRISPR/Cas13a-based nucleic acid detection method for Marburg virus
Full
Kuocheng YAN1, Hao LI1, Xuanyang BAI2, Yao HAN1, Dawei SHI3, Leili JIA2, *, Yansong SUN1, *
Affiliations
  • 1 Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100071, China
  • 2 Chinese PLA Center for Disease Control and Prevention, Beijing 100071, China
  • 3 Division Ⅱ of Diagnostic for Infectious Diseases, National Institutes for Food and Drug Control, Beijing 100050, China
Published: 2024-04-28 doi: 10.13343/j.cnki.wsxb.20240076
Outline
收藏切换

[Objective] To develop a rapid nucleic acid detection method for Marburg virus based on clustered regularly interspaced short palindromic repeats/associated protein 13a (CRISPR/Cas13a). [Methods] According to the conserved region of Marburg virus nucleoprotein (NP) gene, specific primers for reverse transcription recombinase-aided amplification (RT-RAA) and CRISPR RNA (crRNA) were designed and synthesized. RT-RAA was employed to amplify the target sequence. The amplification products were detected by the CRISPR-Cas13a system, and the results were interpreted by easy-readout and sensitive enhanced (ERASE) lateral flow test strips. Finally, the national reference panel was used to evaluate the sensitivity and specificity of the new method. [Results] A set of high-efficiency RT-RAA primers and crRNA targeting Marburg virus NP gene was screened, on the basis of which a CRISPR-ERASE method for the detection of Marburg virus was developed. The target nucleic acid with a concentration of 1 copy/μL could be detected within 1 h, and there was no cross-reaction with other several pathogens. [Conclusion] In this study, a rapid, simple, highly sensitive, and specific nucleic acid detection method for Marburg virus was developed based on CRISPR/Cas13a.

Marburg virus  /  CRISPR/Cas13a  /  nucleic acid detection  /  reverse transcription recombinase-aided amplification (RT-RAA)
Kuocheng YAN, Hao LI, Xuanyang BAI, Yao HAN, Dawei SHI, Leili JIA, Yansong SUN. A CRISPR/Cas13a-based nucleic acid detection method for Marburg virus[J]. Acta Microbiologica Sinica, 2024 , 64 (8) : 3073 -3085 . DOI: 10.13343/j.cnki.wsxb.20240076
Funding
Less
  • National Key Research and Development Program of China(2023YFC2605100)
  • National Key Research and Development Program of China(2021YFC2301100)
Appendix
Less
Year 2024 volume 64 Issue 8
PDF
110
47
Cite this Article
BibTeX
Article Info
doi: 10.13343/j.cnki.wsxb.20240076
  • Receive Date：2024-01-29
  • Online Date：2026-03-19
  • Published：2024-04-28
Article Data
Affiliations
History
  • Received：2024-01-29
  • Accepted：2024-04-19
Funding
National Key Research and Development Program of China(2023YFC2605100)
National Key Research and Development Program of China(2021YFC2301100)
Affiliations
    1 Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100071, China
    2 Chinese PLA Center for Disease Control and Prevention, Beijing 100071, China
    3 Division Ⅱ of Diagnostic for Infectious Diseases, National Institutes for Food and Drug Control, Beijing 100050, China

Corresponding:

*E-mail: JIA Leili,
E-mail: SUN Yansong,
References
Share
https://castjournals.cast.org.cn/joweb/wswxb/EN/10.13343/j.cnki.wsxb.20240076
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House