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Bacillus paralicheniformis, a Gram-positive, facultative anaerobic, motile rod-shaped endospore-forming bacterium, can be used as a species of potential plant growth-promoting rhizobacteria (PGPR). In this study, B. paralicheniformis HMPM220325 was isolated from the fruit fermented milk. This strain can form biofilms at the gas-liquid interface during static cultivation. [Objective] To study the effects of different environmental factors on the biofilm biomass of B. paralicheniformis HMPM220325 and provide data support for the later development and application of HMPM220325 as a PGPR strain. [Methods] Effects of different environmental factors and nutrients on the biofilm formation of B. paralicheniformis HMPM220325 were quantitatively detected by crystal violet staining, and the optimal conditions for the biofilm formation of the strain were optimized by orthogonal experiments. [Results] The optimal environmental conditions for the biofilm formation of B. paralicheniformis HMPM220325 were incubation at 50 ℃ and pH 9.0 for 36 h. The optimal medium was composed of maltose 15.0 g/L, urea 10.0 g/L, magnesium sulphate 20.0 mmol/L, disodium hydrogen phosphate 2.5 g/L, and bovine heart infusion 17.5 g/L. The optimized culture conditions increased the biofilm biomass by 58.28% compared with the original culture conditions. [Conclusion] This study explored the biofilm formation of B. paralicheniformis in a variety of environments and optimized the culture conditions for biofilm formation of this strain, providing an experimental basis for further development of PGPR.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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