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Construction and immunogenicity of serotype O recombinant foot-and-mouth disease virus
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Shulun HUANG1, 2, Jingjing ZHA1, 2, Pu SUN1, 2, Xingze ZHANG1, 2, Dong LI1, 2, Yimei CAO1, 2, Xingwen BAI1, 2, Yuanfang FU1, 2, Xueqing MA1, 2, Kun LI1, 2, Hong YUAN1, 2, Zengjun LU1, 2, Zaixin LIU1, 2, Pinghua LI1, 2, *
Acta Microbiologica Sinica | 2024, 64(7) : 2493 - 2501
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Acta Microbiologica Sinica | 2024, 64(7): 2493-2501
• Research Articles •
Construction and immunogenicity of serotype O recombinant foot-and-mouth disease virus
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Shulun HUANG1, 2, Jingjing ZHA1, 2, Pu SUN1, 2, Xingze ZHANG1, 2, Dong LI1, 2, Yimei CAO1, 2, Xingwen BAI1, 2, Yuanfang FU1, 2, Xueqing MA1, 2, Kun LI1, 2, Hong YUAN1, 2, Zengjun LU1, 2, Zaixin LIU1, 2, Pinghua LI1, 2, *
Affiliations
  • 1 State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, Gansu, China
  • 2 Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou 730046, Gansu, China
Published: 2024-07-04 doi: 10.13343/j.cnki.wsxb.20230803
Outline
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[Objective] To construct a recombinant food-and-mouth disease virus (FMDV) strain carrying the genes encoding three topotypes of immunodominant structural proteins of serotype O FMDV by reverse genetic manipulation and evaluate the potential of the recombinant strain serving as a vaccine candidate for porcine food-and-mouth disease (FMD) type O. [Methods] Based on the gene of the recombinant FMDV with the replacement of the VP1 structural protein of O/NXYCh/CHA/2018 epidemic strain, the recombinant full-length plasmid featuring substitution of G-H loop genes of the structural protein VP1 of O/TUR/5/2009 vaccine strain was constructed by gene synthesis. The recombinant virus was rescued after transfection of the linearized recombinant plasmid into BSR/T7 cells expressing T7 RNA polymerase, and then identified by RT-PCR, sequencing, and indirect immunofluorescence. The plaque assay and one-step growth curve building were employed to characterize the recombinant virus. Pigs were vaccinated with the vaccines prepared from the recombinant virus and the parental virus, and then virus neutralization tests were carried out to examine the cross-reactive responses against the epidemic serotype O FMDV isolates of three topotypes. [Results] The recombinant FMDV strain carrying the structural protein genes of three topotypes was successful rescued. The recombinant strain showed similar biological properties to the parental virus. Pigs vaccinated with the vaccines prepared from the recombinant virus and the parental virus produced protective neutralizing antibodies with the mean titer of > 1.65log10 against the viruses of the Middle East-South Asia (ME-SA) and South-East Asia (SEA) topotypes. The pigs did not produce protective neutralizing antibodies against the Cathay topotype (< 1.65log10). The substitution of O/TUR/5/2009 G-H loop gene improved the cross-reactivity against the viruses of ME-SA and SEA topotypes compared with the parental virus (P < 0.05). [Conclusion] This study has guiding significance for the design of FMD vaccines in the future.

foot-and-mouth disease (FMD)  /  recombinant virus  /  construction  /  immunogenicity
Shulun HUANG, Jingjing ZHA, Pu SUN, Xingze ZHANG, Dong LI, Yimei CAO, Xingwen BAI, Yuanfang FU, Xueqing MA, Kun LI, Hong YUAN, Zengjun LU, Zaixin LIU, Pinghua LI. Construction and immunogenicity of serotype O recombinant foot-and-mouth disease virus[J]. Acta Microbiologica Sinica, 2024 , 64 (7) : 2493 -2501 . DOI: 10.13343/j.cnki.wsxb.20230803
Funding
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  • National Key Research and Development Program of China(2023YFD1802501)
Appendix
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Year 2024 volume 64 Issue 7
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Article Info
doi: 10.13343/j.cnki.wsxb.20230803
  • Receive Date：2023-12-27
  • Online Date：2026-03-19
  • Published：2024-07-04
Article Data
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History
  • Received：2023-12-27
  • Accepted：2024-04-22
Funding
National Key Research and Development Program of China(2023YFD1802501)
Affiliations
    1 State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, Gansu, China
    2 Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou 730046, Gansu, China

Corresponding:

*LI Pinghua, E-mail:
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表12种不同金属材料的力学参数
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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