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Screening of phosphorus-solubilizing strains with stress tolerance in the permafrost region of the Qinghai-Xizang Plateau
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Hanyu ZHNG1, 2, Dandan LI1, *, Jin ZHENG1, Jinjuan FAN2, Qingwei WANG1, Xianyuan DU1, Jinman REN3, Quanwei SONG1, Huijun WU1, Jiacai XIE1
Acta Microbiologica Sinica | 2024, 64(6) : 1876 - 1890
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Acta Microbiologica Sinica | 2024, 64(6): 1876-1890
• Microbiome in Extreme Environments •
Screening of phosphorus-solubilizing strains with stress tolerance in the permafrost region of the Qinghai-Xizang Plateau
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Hanyu ZHNG1, 2, Dandan LI1, *, Jin ZHENG1, Jinjuan FAN2, Qingwei WANG1, Xianyuan DU1, Jinman REN3, Quanwei SONG1, Huijun WU1, Jiacai XIE1
Affiliations
  • 1 State Key Laboratory of Petroleum Pollution Control, China National Petroleum Corporation Research Institute of Safety and Environment Technology, Beijing 102206, China
  • 2 College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, Liaoning, China
  • 3 Daqing Oilfield Water and Environmental Protection Company, Daqing 163712, Heilongjiang, China
Published: 2024-06-04 doi: 10.13343/j.cnki.wsxb.20230677
Outline
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[Objective] To isolate efficient phosphorus-solubilizing strains from soils in the permafrost region of the Qinghai-Xizang Plateau and provide strain resources for the activation of unavailable phosphorus in the soil of the Plateau. [Methods] The selective media with organic and inorganic phosphorus were used to isolate phosphorus-solubilizing strains from the soil samples collected in the permafrost region around the Tanggula Pass of the Qinghai-Xizang Plateau by the plate streaking method. The phylogenetic tree was built based on the 16S rRNA gene sequences to identify the strains, and then the phosphorus-solubilizing abilities and stress tolerance of the strains were determined. [Results] Five strains belonging toPseudomonas were isolated, including three inorganic phosphorus-solubilizing strains (i5, i6, and i9L) and two organic phosphorus-solubilizing strains (Qb and Qo). After 7 days of incubation in shake flasks at 30 ℃, the content of available phosphorus in the supernatants of Qb and Qo was 534.8 mg/L and 723.7 mg/L, respectively, which was significantly higher than that (166.9–210.5 mg/L) in the supernatants of i5, i6, and i9L. The available phosphorus content in the supernatant of Qo was the highest (519.7–683.0 mg/L) among the five isolates under different concentrations of PEG6000. The phosphorus-solubilizing abilities of Qb and Qo were stronger than those of the other strains at 5 ℃ and 10 ℃. [Conclusion] Qo outperformed the other strains in terms of tolerance to low temperature and drought stress, serving the development of microbial fertilizers and vegetation restoration in alpine regions such as the Qinghai-Xizang Plateau.

Qinghai-Xizang Plateau  /  permafrost region  /  phosphorus-solubilizing strain  /  low temperature stress  /  drought stress
Hanyu ZHNG, Dandan LI, Jin ZHENG, Jinjuan FAN, Qingwei WANG, Xianyuan DU, Jinman REN, Quanwei SONG, Huijun WU, Jiacai XIE. Screening of phosphorus-solubilizing strains with stress tolerance in the permafrost region of the Qinghai-Xizang Plateau[J]. Acta Microbiologica Sinica, 2024 , 64 (6) : 1876 -1890 . DOI: 10.13343/j.cnki.wsxb.20230677
Funding
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  • Scientific Research and Technology Development Program of China National Petroleum Corporation Research Institute of Safety and Environment Technology(RISE2022KY08)
  • China Postdoctoral Science Foundation(2022M713468)
  • Science and Technology Program of China National Petroleum Corporation(2023ZZ1303)
  • Basic Science and Strategic Reserves Technology Research Fund Program of China National Petroleum Corporation(2022DQ03-A5)
Appendix
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Year 2024 volume 64 Issue 6
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Article Info
doi: 10.13343/j.cnki.wsxb.20230677
  • Receive Date：2023-11-03
  • Online Date：2026-03-19
  • Published：2024-06-04
Article Data
Affiliations
History
  • Received：2023-11-03
  • Accepted：2024-04-17
Funding
Scientific Research and Technology Development Program of China National Petroleum Corporation Research Institute of Safety and Environment Technology(RISE2022KY08)
China Postdoctoral Science Foundation(2022M713468)
Science and Technology Program of China National Petroleum Corporation(2023ZZ1303)
Basic Science and Strategic Reserves Technology Research Fund Program of China National Petroleum Corporation(2022DQ03-A5)
Affiliations
    1 State Key Laboratory of Petroleum Pollution Control, China National Petroleum Corporation Research Institute of Safety and Environment Technology, Beijing 102206, China
    2 College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, Liaoning, China
    3 Daqing Oilfield Water and Environmental Protection Company, Daqing 163712, Heilongjiang, China

Corresponding:

*LI Dandan, E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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