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Bacillus velezensis B6: mechanism of enhanced oil recovery and application in highly mineralized oil reservoirs
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Xianke CHEN1, 2, 7, Peiyao SUN3, *, Yong CAI4, Miaofeng ZHANG5, Wei ZHANG5, Bo YANG6, Anzhou MA1, 7
Acta Microbiologica Sinica | 2024, 64(6) : 1948 - 1959
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Acta Microbiologica Sinica | 2024, 64(6): 1948-1959
• Geomicrobiological Applications •
Bacillus velezensis B6: mechanism of enhanced oil recovery and application in highly mineralized oil reservoirs
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Xianke CHEN1, 2, 7, Peiyao SUN3, *, Yong CAI4, Miaofeng ZHANG5, Wei ZHANG5, Bo YANG6, Anzhou MA1, 7
Affiliations
  • 1 Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
  • 2 Sino-Danish College, University of Chinese Academy of Sciences, Beijing 101400, China
  • 3 Drilling and Production Technology Research Institute of Qinghai Oilfield Company, Dunhuang 736202, Gansu, China
  • 4 The Fifth Oil Production Plant of Qinghai Oilfield Company, Mangya 816400, Qinghai, China
  • 5 The Third Oil Production Plant of Qinghai Oilfield Company, Mangya 816400, Qinghai, China
  • 6 The First Oil Production Plant of Qinghai Oilfield Company, Mangya 816400, Qinghai, China
  • 7 University of Chinese Academy of Sciences, Beijing 100049, China
Published: 2024-06-04 doi: 10.13343/j.cnki.wsxb.20230560
Outline
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[Objective] To screen the indigenous functional microorganisms in reservoirs and explore their oil recovery mechanisms, we conducted field tests to determine the process and technical feasibility of enhancing crude oil recovery by indigenous microorganisms. [Methods] We collected samples from the Yingdong Oilfield and used oil plates to screen for the target bacteria. Next, we evaluated the environmental adaptability and optimized the culture conditions of the strain. Further, we measured the emulsifying, viscosity-reducing, paraffin-resistant, and hydrocarbon conversion abilities of the isolate to evaluate the oil recovery competence of the strain and explored its oil recovery mechanism. Finally, we carried out the microbial enhanced oil recovery tests in the oilfields. [Results] We isolatedBacillus velezensis B6 from the oil-water sample with an emulsifying activity index (EI24) of 100.00%, a viscosity reduction rate of 97.20%, and a paraffin resistance rate of 86.90%, which indicated that strain B6 had good emulsifying and viscosity-reducing abilities and improved oil recovery potential. Strain B6 could reduce heavy components in crude oil and increase light hydrocarbons to improve crude oil properties and quality. Moreover, we carried out single-well huff and puff and paraffin removal operations in Yingdong Oilfield, Yuejin Oilfield, and Huatugou Oilfield with a total of 62 well-times in the field test, which resulted in a cumulative oil increase of 1 460.36 tons and an average delay of 47 days in the well flushing cycle. The economic benefit was CNY 3.425 million, and the input-output ratio was 1:4. [Conclusion] The laboratory studies and field tests proved that indigenousB.velezensis B6 can significantly improve the oil recovery and well paraffin removal, with great application potential.

microbial huff and puff  /  emulsification and viscosity reduction  /  large-scale fermentation  /  microbial paraffin removal
Xianke CHEN, Peiyao SUN, Yong CAI, Miaofeng ZHANG, Wei ZHANG, Bo YANG, Anzhou MA. Bacillus velezensis B6: mechanism of enhanced oil recovery and application in highly mineralized oil reservoirs[J]. Acta Microbiologica Sinica, 2024 , 64 (6) : 1948 -1959 . DOI: 10.13343/j.cnki.wsxb.20230560
Funding
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  • National Key Research and Development Program of China(2018YFA0901200)
  • Extension and Application of Microbial Oil Recovery Technology in Qinghai Oilfield(2022T01)
  • Qinghai Science and Technology Plan(2022-QY-202)
Appendix
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Year 2024 volume 64 Issue 6
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65
24
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Article Info
doi: 10.13343/j.cnki.wsxb.20230560
  • Receive Date：2023-09-01
  • Online Date：2026-03-19
  • Published：2024-06-04
Article Data
Affiliations
History
  • Received：2023-09-01
  • Accepted：2023-11-10
Funding
National Key Research and Development Program of China(2018YFA0901200)
Extension and Application of Microbial Oil Recovery Technology in Qinghai Oilfield(2022T01)
Qinghai Science and Technology Plan(2022-QY-202)
Affiliations
    1 Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
    2 Sino-Danish College, University of Chinese Academy of Sciences, Beijing 101400, China
    3 Drilling and Production Technology Research Institute of Qinghai Oilfield Company, Dunhuang 736202, Gansu, China
    4 The Fifth Oil Production Plant of Qinghai Oilfield Company, Mangya 816400, Qinghai, China
    5 The Third Oil Production Plant of Qinghai Oilfield Company, Mangya 816400, Qinghai, China
    6 The First Oil Production Plant of Qinghai Oilfield Company, Mangya 816400, Qinghai, China
    7 University of Chinese Academy of Sciences, Beijing 100049, China

Corresponding:

*SUN Peiyao, Tel: +86-937-8920426, E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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