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Prokaryotic expression ofMycoplasmabovis LRR5 protein involved in the invasion ofMycoplasmabovis into host cells
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Yaru FENG1, 2, Mengting ZHOU1, 2, Yuyu ZHANG1, 2, Ge ZHANG1, 2, Pengcheng GAO3, 4, Duoliang RAN1, 2, Jian XU1, 3, 4, Yuefeng CHU1, 3, 4, *, Bin LI1, 2, *
Acta Microbiologica Sinica | 2024, 64(5) : 1469 - 1482
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Acta Microbiologica Sinica | 2024, 64(5): 1469-1482
• Research Articles •
Prokaryotic expression ofMycoplasmabovis LRR5 protein involved in the invasion ofMycoplasmabovis into host cells
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Yaru FENG1, 2, Mengting ZHOU1, 2, Yuyu ZHANG1, 2, Ge ZHANG1, 2, Pengcheng GAO3, 4, Duoliang RAN1, 2, Jian XU1, 3, 4, Yuefeng CHU1, 3, 4, *, Bin LI1, 2, *
Affiliations
  • 1 College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
  • 2 Xinjiang Key Laboratory of Research and Creation of New Drugs for Herbivores, Urumqi 830052, Xinjiang, China
  • 3 State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, Gansu, China
  • 4 Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou 730046, Gansu, China
Published: 2024-05-04 doi: 10.13343/j.cnki.wsxb.20230688
Outline
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Pathogenic mycoplasma has the ability to invade host cells, which is a key mechanism to their pathogenicity. Functional proteins that mediate the entry of mycoplasma may serve as potential drug or vaccine targets. [Objective] To clone and express the LRR5 protein encoded byMBOVPG45_0564 inMycoplasmabovis and to explore its role in the invasion of host cells byM.bovis. [Methods] The NCBI database was used for the homology analysis ofMBOVPG45_0564, and the structure of LRR5 protein was predicted by the Discovery Studio Client system. After prokaryotic expression of LRR5 protein, the mouse polyclonal antibody was prepared, and the subcellular localization of LRR5 protein was observed by immunoelectron microscopy. The invasion ofM.bovis into embryonic bovine lung (EBL) cells after LRR5 antibody blocking was observed by plate counting and laser confocal microscopy. After conjugation of LRR5 protein to the surface of fluorescent microspheres, the entry of the microspheres into EBL cells was observed by laser confocal microscopy and a high-content live-cell imaging system. [Results] MBOVPG45_0564 was a conserved sequence inM.bovis and encoded LRR5, a membrane-associated protein with a typical crescent-like spatial conformation. Multiple repeating leucine motifs were assembled in a supercoiled manner to form a solenoid protein structural unit. After LRR5 antibody blocking, the invasion rate ofM.bovis to EBL cells reduced (P<0.05), and the fluorescent microspheres conjugated with LRR5 protein could successfully enter EBL cells. [Conclusion] The LRR5 protein encoded byMBOVPG45_0564 is localized on theM.bovis membrane and plays a role in the invasion ofM.bovis into host cells.

Mycoplasmabovis  /  MBOVPG45_0564  /  invasion  /  protein expression  /  biological function
Yaru FENG, Mengting ZHOU, Yuyu ZHANG, Ge ZHANG, Pengcheng GAO, Duoliang RAN, Jian XU, Yuefeng CHU, Bin LI. Prokaryotic expression ofMycoplasmabovis LRR5 protein involved in the invasion ofMycoplasmabovis into host cells[J]. Acta Microbiologica Sinica, 2024 , 64 (5) : 1469 -1482 . DOI: 10.13343/j.cnki.wsxb.20230688
Funding
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  • National Natural Science Foundation of China(32260885)
  • Open Project of Key Laboratory of "Research and Creation of New Drugs for Herbivores in Xinjiang"(XJCDVM-HDRC-S202305)
  • Key Task Project of Innovation Engineering of Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences(CAAS-ASTIP-JBGS-20210701)
  • Major Science and Technology Project of Gansu Province(22ZD6NA001)
  • Research and Innovation Projects for University Students(dxscx2023196)
Appendix
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Year 2024 volume 64 Issue 5
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56
21
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Article Info
doi: 10.13343/j.cnki.wsxb.20230688
  • Receive Date：2023-11-08
  • Online Date：2026-03-19
  • Published：2024-05-04
Article Data
Affiliations
History
  • Received：2023-11-08
  • Accepted：2024-02-19
Funding
National Natural Science Foundation of China(32260885)
Open Project of Key Laboratory of "Research and Creation of New Drugs for Herbivores in Xinjiang"(XJCDVM-HDRC-S202305)
Key Task Project of Innovation Engineering of Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences(CAAS-ASTIP-JBGS-20210701)
Major Science and Technology Project of Gansu Province(22ZD6NA001)
Research and Innovation Projects for University Students(dxscx2023196)
Affiliations
    1 College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
    2 Xinjiang Key Laboratory of Research and Creation of New Drugs for Herbivores, Urumqi 830052, Xinjiang, China
    3 State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, Gansu, China
    4 Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou 730046, Gansu, China

Corresponding:

*CHU Yuefeng, E-mail:;
LI Bin, E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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