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Polysaccharide hydrolysis activity and genomic information ofSaccharophagus degradans FZY0027
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Ziyue FU1, Daofeng ZHANG1, *, Menghan HUANG1, Jinglin LI1, Haochen SU1, Wenjun LI1, 2, 3, *
Acta Microbiologica Sinica | 2024, 64(5) : 1593 - 1606
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Acta Microbiologica Sinica | 2024, 64(5): 1593-1606
• Research Articles •
Polysaccharide hydrolysis activity and genomic information ofSaccharophagus degradans FZY0027
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Ziyue FU1, Daofeng ZHANG1, *, Menghan HUANG1, Jinglin LI1, Haochen SU1, Wenjun LI1, 2, 3, *
Affiliations
  • 1 Institute of Marine Biotechnology and Bio-resource Utilization, College of Oceanography, Hohai University, Nanjing 210098, Jiangsu, China
  • 2 State Key Laboratory of Pest Biocontrol and Resource Utilization, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, Guangdong, China
  • 3 State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, Xinjiang, China
Published: 2024-05-04 doi: 10.13343/j.cnki.wsxb.20230764
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[Objective] To analyze the polysaccharide hydrolysis activity and genomic characteristics of a Gram-negative bacterial strain FZY0027 isolated from intertidal seawater. [Methods] The strain FZY0027 was identified based on the morphological characteristics, 16S rRNA gene sequence, and the whole genome sequence determined by Illumina NovaSeq and Oxford Nanopore PromethION. Bioinformatics tools such as dbCAN, EasyCGTree, BRIG, and Easyfig were used to compare the strain FZY0027 withSaccharophagus degradans 2-40T. The 3, 5-dinitrosalicylic acid (DNS) method was employed to measure the polysaccharide hydrolysis activity of strain FZY0027. [Results] The 16S rRNA gene sequence showed the similarity of 99.9% between strain FZY0027 andS.degradans 2-40T, and thus strain FZY0027 was preliminarily identified asS.degradans FZY0027. The highest levels of reducing sugars (2.28, 1.75, and 1.10 mg/mL, respectively) were produced by FZY0027 through the hydrolysis of starch, xylan, and mannose. The genome of strain FZY0027 was 5 178 381 bp, encoding a total of 4 156 genes, with the G+C content of 45.8%. The average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values between strain FZY0027 andS.degradans 2-40T were 96.5%, 96.7%, and 70.0%, respectively. A total of 303 genes were annotated in the Carbohydrate-Active Enzyme database, and there was a significant difference in the number (137 and 130, respectively) of genes encoding glycoside hydrolases (GHs) between strain FZY0027 andS.degradans 2-40T. Strain FZY0027 carried multiple genes involved in the hydrolysis of starch and xylan, which was corresponding to its strong ability to hydrolyse starch and xylan. However, compared withS.degradans 2-40T, strain FZY0027 could only hydrolyse a few polysaccharides under the experimental conditions in this study, which suggested that this strain may require specific culture conditions to fully exert its polysaccharide hydrolysis ability. [Conclusion] Strain FZY0027 is a versatile polysaccharide-hydrolyzing bacterium with the potential for bioresource utilization.

Saccharophagus degradans  /  polysaccharide hydrolysis activity  /  genome  /  bioinformatics analysis
Ziyue FU, Daofeng ZHANG, Menghan HUANG, Jinglin LI, Haochen SU, Wenjun LI. Polysaccharide hydrolysis activity and genomic information ofSaccharophagus degradans FZY0027[J]. Acta Microbiologica Sinica, 2024 , 64 (5) : 1593 -1606 . DOI: 10.13343/j.cnki.wsxb.20230764
Funding
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  • National Natural Science Foundation of China(31900001)
  • Innovation Project for Marine Science and Technology of Jiangsu Province(JSZRHYKJ202209)
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Year 2024 volume 64 Issue 5
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Article Info
doi: 10.13343/j.cnki.wsxb.20230764
  • Receive Date：2023-12-12
  • Online Date：2026-03-19
  • Published：2024-05-04
Article Data
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History
  • Received：2023-12-12
  • Accepted：2024-02-07
Funding
National Natural Science Foundation of China(31900001)
Innovation Project for Marine Science and Technology of Jiangsu Province(JSZRHYKJ202209)
Affiliations
    1 Institute of Marine Biotechnology and Bio-resource Utilization, College of Oceanography, Hohai University, Nanjing 210098, Jiangsu, China
    2 State Key Laboratory of Pest Biocontrol and Resource Utilization, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, Guangdong, China
    3 State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, Xinjiang, China

Corresponding:

*LI Wenjun, E-mail:;
ZHANG Daofeng, E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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