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Phosphate-responsive promoter region inHeyndrickxia coagulans
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Xiaoxiao QI1, 2, Limin WANG1, Bo YU1, *
Acta Microbiologica Sinica | 2024, 64(5) : 1538 - 1549
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Acta Microbiologica Sinica | 2024, 64(5): 1538-1549
• Research Articles •
Phosphate-responsive promoter region inHeyndrickxia coagulans
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Xiaoxiao QI1, 2, Limin WANG1, Bo YU1, *
Affiliations
  • 1 Department of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
  • 2 University of Chinese Academy of Sciences, Beijing 100049, China
Published: 2024-05-04 doi: 10.13343/j.cnki.wsxb.20230720
Outline
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Heyndrickxia coagulans characterized by low nutrient requirements, high titers of fermentation products, and thermal tolerance has become a major microbial species for lactic acid fermentation. We have demonstrated that phosphate stimulates the gene expression of L-lactate dehydrogenase inH.coagulans to increase L-lactic acid production, the mechanism of which, however, remains unknown. [Objective] To primarily investigated the mechanism of phosphate in stimulating the gene transcription of lactate dehydrogenase inH.coagulans. [Methods] RT-PCR was employed to analyze the transcriptional level changes of lactate dehydrogenase inH.coagulans after phosphate addition. The core promotor region responsive to phosphate stimulation was identified by conventional methods of molecular biology. [Results] The key element responsive to phosphate was located in the upstream promoter region of the L-lactate dehydrogenase gene. The core region of the promoter was responsible for phosphate stimulation. The identified promoter was employed to promote D-lactate production with diammonium phosphate addition. [Conclusion] This study reported a new phosphate-responsive gene element, providing a theoretical basis for improving the synthesis efficiency of other biochemicals.

Heyndrickxia coagulans  /  phosphate-responsive  /  promoter  /  lactate dehydrogenase
Xiaoxiao QI, Limin WANG, Bo YU. Phosphate-responsive promoter region inHeyndrickxia coagulans[J]. Acta Microbiologica Sinica, 2024 , 64 (5) : 1538 -1549 . DOI: 10.13343/j.cnki.wsxb.20230720
Funding
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  • National Natural Science Foundation of China(31970025)
  • National Natural Science Foundation of China(32070026)
  • Inner Mongolia Autonomous Region Research Project of China(2022JBGS0007)
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Year 2024 volume 64 Issue 5
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Article Info
doi: 10.13343/j.cnki.wsxb.20230720
  • Receive Date：2023-11-26
  • Online Date：2026-03-19
  • Published：2024-05-04
Article Data
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History
  • Received：2023-11-26
  • Accepted：2024-02-19
Funding
National Natural Science Foundation of China(31970025)
National Natural Science Foundation of China(32070026)
Inner Mongolia Autonomous Region Research Project of China(2022JBGS0007)
Affiliations
    1 Department of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
    2 University of Chinese Academy of Sciences, Beijing 100049, China

Corresponding:

*YU Bo, E-mail:
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Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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